Heterogeneity of radiation response of a parent human epidermoid carcinoma cell line and four clones.

We examined the radiobiological parameters of a parent tumor cell line and four tumor clones of a human skin squamous cell carcinoma. The parent line and all clones have a tumor morphology, aneuploid karyotype, and the ability to passage infinitely in vitro. The parent cell line and three of four clones formed tumors in nude mice. The parent line, SCC-12, has a D0 of 1.59 Gy and an n of 7.5. In the four clones, D0 ranges from 1.31 Gy to 2.66 Gy and n ranges from 2.1 to 22.8. Potentially lethal damage repair (PLDR) following 11 Gy ranges from 1.7 to 13.1, at 24 hours, however PLDR following equitoxic doses of radiation is similar. The mean inactivation dose value (D) in the parent tumor cell line is 3.48 and ranges from 3.31 to 4.17 in the tumor clones. Radiobiological interpretation of heterogeneity may complicate predictive assays for clinical radiotherapy.

[1]  M. M. ELKIND,et al.  X-Ray Damage and Recovery in Mammalian Cells in Culture , 1959, Nature.

[2]  R. Weichselbaum,et al.  Radiation-resistant and repair-proficient human tumor cells may be associated with radiotherapy failure in head- and neck-cancer patients. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[3]  I. Fidler,et al.  Metastasis results from preexisting variant cells within a malignant tumor. , 1977, Science.

[4]  F. Miller,et al.  Heterogeneity in drug sensitivity among tumor cell subpopulations of a single mammary tumor. , 1978, Cancer research.

[5]  F. Kwong,et al.  Radiation and melanoma: response of B16 mouse tumor cells and clonal lines to in vitro irradiation. , 1979, Radiation research.

[6]  R. Weichselbaum,et al.  The maximum recovery potential of human tumor cells may predict clinical outcome in radiotherapy. , 1987, International journal of radiation oncology, biology, physics.

[7]  J. Dewyngaert,et al.  Differential responses to x-irradiation of subpopulations of two heterogeneous human carcinomas in vitro. , 1982, Cancer research.

[8]  R. Prehn.  Analysis of antigenic heterogeneity within individual 3-methylcholanthrene-induced mouse sarcomas. , 1970, Journal of the National Cancer Institute.

[9]  J. Leith,et al.  Effects of nutritional state on the expression of radiation injury in two tumor subpopulations obtained from a heterogeneous human colon carcinoma. , 1986, International journal of radiation oncology, biology, physics.