IDENTIFICATION OF AND DETECTION OF ITS AFLATOXIN GENES ISOLATED FROM PEANUT

is one of the main fungi that are able to produce aflatoxin. The presence of the fungi and its aflatoxin become serious problem on food safety. This research was aimed to isolate and identify from peanut and its processed products collected from some traditional markets in Bogor, Depok and Jakarta, and detection of their aflatoxin genes. Fungal isolation was using AFPA media. Fungal identification was carried out by combining morphological and molecular analysis using species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R,whiledetectionof aflatoxingenesemployedfourspecificprimersof ( ), ( ), ( ) and ( ). From 36 samples, the group was only found in peanut kernels samples with viable count of specific colonies in the range of 0.01-5.52 x 10 cfu/g. The total of 18 isolates were identified as based on species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R by producing amplicons about 100 and 413 bp, respectively. Based on aflatoxin gene analysis it was showed that all 18 isolates was successfully amplified by both and , 83.3 % by and 72.2 % by genes which taking part in aflatoxin production. The amplicons size of and primer pairs were about1032,400,895and1024bp,respectively.

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