Characteristics of subgingival calculus detection by multiphoton fluorescence microscopy.

Subgingival calculus has been recognized as a major cause of periodontitis, which is one of the main chronic infectious diseases of oral cavities and a principal cause of tooth loss in humans. Bacteria deposited in subgingival calculus or plaque cause gingival inflammation, function deterioration, and then periodontitis. However, subgingival calculus within the periodontal pocket is a complicated and potentially delicate structure to be detected with current dental armamentaria, namely dental x-rays and dental probes. Consequently, complete removal of subgingival calculus remains a challenge to periodontal therapies. In this study, the detection of subgingival calculus employing a multiphoton autofluorescence imaging method was characterized in comparison with a one-photon confocal fluorescence imaging technique. Feasibility of such a system was studied based on fluorescence response of gingiva, healthy teeth, and calculus with and without gingiva covered. The multiphoton fluorescence technology perceived the tissue-covered subgingival calculus that cannot be observed by the one-photon confocal fluorescence method.

[1]  P. So,et al.  Cellular response to near-infrared femtosecond laser pulses in two-photon microscopes. , 1997, Optics letters.

[2]  Tatsuji Nishihara,et al.  Detection of subgingival calculus and dentine caries by laser fluorescence. , 2004 .

[3]  Poul Erik Petersen,et al.  The World Oral Health Report 2003: continuous improvement of oral health in the 21st century--the approach of the WHO Global Oral Health Programme. , 2003, Community dentistry and oral epidemiology.

[4]  Albert Mehl,et al.  Subgingival calculus detection with fluorescence induced by 655 nm InGaAsP diode laser radiation. , 2002, Journal of periodontology.

[5]  P. Hirschmann,et al.  The usefulness of radiographs in diagnosis and management of periodontal diseases: a review. , 2000, Journal of dentistry.

[6]  J. Pratten,et al.  Study of bacterial viability within human supragingival dental calculus. , 2004, Journal of periodontology.

[7]  M. Frentzen,et al.  The possibility of detecting subgingival calculus by laser-fluorescence in vitro , 2003, Lasers in Medical Science.

[8]  V Clerehugh,et al.  Subgingival calculus: where are we now? A comparative review. , 2000, Journal of dentistry.

[9]  G. Somesfalean,et al.  Real-time detection of dental calculus by blue-LED-induced fluorescence spectroscopy. , 2007, Journal of photochemistry and photobiology. B, Biology.

[10]  K. Fujita.  [Two-photon laser scanning fluorescence microscopy]. , 2007, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme.

[11]  P. Petersen.  [Continuous improvement of oral health in the 21st century: the approach of the WHO Global Oral Health Programme]. , 2004, Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology.

[12]  Albert Mehl,et al.  The effectiveness of InGaAsP diode laser radiation to detect subgingival calculus as compared to an explorer. , 2004, Journal of periodontology.

[13]  P. R. Sherman,et al.  The effectiveness of subgingival scaling and root planning. I. Clinical detection of residual calculus. , 1990, Journal of periodontology.

[14]  K L Vandana,et al.  Thickness of gingiva in association with age, gender and dental arch location. , 2005, Journal of clinical periodontology.

[15]  Y. Lai,et al.  Detection of subgingival calculus through oral gum in vitro using two-photon fluorescence microscopy. , 2008, Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference.

[16]  R. Wong,et al.  Tooth dimensions of Southern Chinese. , 2007, Homo : internationale Zeitschrift fur die vergleichende Forschung am Menschen.

[17]  J. Lindhe,et al.  Textbook of clinical periodontology , 1989 .

[18]  Walter J. Riker.  A Review of J , 2010 .

[19]  A. Sukhinina,et al.  Fluorescence spectroscopy of dental calculus , 2010 .