STIMULATION EFFECT OF SILVER NITRATE ON SHOOT REGENERATION IN COTYLEDON TISSUE CULTURE OF BRASSIA CAMPESTRIS

A tissue culture system for obtaining high frequency shoot regeneration of Brassica campestris was studied. Shoots could be induced directly from cotyledons cultured on MS medium supplemented with 2.0 mg L~(-1) BA and 0.5 mg L~(-1) NAA, the shoot regeneration rate being 31.6%. Addition of 2.0 mg L~(-1) AgNO_(3) in the medium could greatly increase the frequency of shoot regeneration, and the shoot regeneration rate reached to 86.5%.