Effect of High-Dose Nano-selenium and Selenium–Yeast on Feed Digestibility, Rumen Fermentation, and Purine Derivatives in Sheep

The aim of this study was to evaluate the effect of nano-selenium (NS) and yeast–selenium (YS) supplementation on feed digestibility, rumen fermentation, and urinary purine derivatives in sheep. Six male ruminally cannulated sheep, average 43.32 ± 4.8 kg of BW, were used in a replicated 3 × 3 Latin square experiment. The treatments were control (without NS and YS), NS with 4 g nano-Se (provide 4 mg Se), and YS with 4 g Se-yeast (provide 4 mg Se) per kilogram of diet dry matter (DM), respectively. Experimental periods were 25 days with 15 days of adaptation and 10 days of sampling. Ruminal pH, ammonia N concentration, molar proportion of propionate, and ratio of acetate to propionate were decreased (P < 0.01), and total ruminal VFA concentration was increased with NS and YS supplementation (P < 0.01). In situ ruminal neutral detergent fiber (aNDF) degradation of Leymus chinensis (P < 0.01) and crude protein (CP) of soybean meal (P < 0.01) were significantly improved by Se supplementation. Digestibilities of DM, organic matter, crude protein, ether extract, aNDF, and ADF in the total tract and urinary excretion of purine derivatives were also affected by feeding Se supplementation diets (P < 0.01). Ruminal fermentation was improved by feeding NS, and feed conversion efficiency was also increased compared with YS (P < 0.01). We concluded that nano-Se can be used as a preferentially available selenium source in ruminant nutrition.

[1]  P. V. Soest,et al.  A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability. , 1992, Journal of animal science.

[2]  J. Néve,et al.  Selenium as a ‘nutraceutical’: how to conciliate physiological and supra-nutritional effects for an essential trace element , 2002, Current opinion in clinical nutrition and metabolic care.

[3]  X. B. Chen,et al.  ESTIMATION OF MICROBIAL PROTEIN SUPPLY TO SHEEP AND CATTLE BASED ON URINARY EXCRETION OF PURINE DERIVATIVES - AN OVERVIEW OF THE TECHNICAL DETAILS , 1995 .

[4]  Li Lijia,et al.  Cytogenetic mapping of disease resistance genes and analysis of their distribution features on chromosomes in maize , 2003 .

[5]  A. J. Barr,et al.  SAS user's guide , 1979 .

[6]  G. Jiang,et al.  Elemental selenium particles at nano-size (Nano-Se) are more toxic to Medaka (Oryzias latipes) as a consequence of hyper-accumulation of selenium: a comparison with sodium selenite. , 2008, Aquatic toxicology.

[7]  A. Pechová,et al.  Monitoring of Changes in Selenium Concentration in Goat Milk During Short-Term Supplementation of Various Forms of Selenium , 2008, Biological Trace Element Research.

[8]  Li-guang Shi,et al.  Effect of elemental nano-selenium on feed digestibility, rumen fermentation, and purine derivatives , 2011 .

[9]  Gregory J. Exarhos,et al.  SYNTHESIS AND STABILIZATION OF SELENIUM NANOPARTICLES ON CELLULOSE NANOCRYSTAL , 2007 .

[10]  P. Whanger.  Selenocompounds in Plants and Animals and their Biological Significance , 2002, Journal of the American College of Nutrition.

[11]  I. McDonald.  A revised model for the estimation of protein degradability in the rumen , 1981, The Journal of Agricultural Science.

[12]  A. Garg,et al.  Effect of Organic Selenium Supplementation on Growth, Se Uptake, and Nutrient Utilization in Guinea Pigs , 2010, Biological Trace Element Research.

[13]  R. C. Ewan.  Effect of selenium on rat growth, growth hormone and diet utilization. , 1976, The Journal of nutrition.

[14]  R. Dass,et al.  Selenium supplementation influences growth performance, antioxidant status and immune response in lambs , 2009 .

[15]  E. R. Ørskov,et al.  The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage , 1979, The Journal of Agricultural Science.

[16]  Ľ. Grešáková,et al.  The effects of organic selenium supplementation on the rumen ciliate population in sheep , 2008, Folia Microbiologica.

[17]  Hanqing Yu,et al.  Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice. , 2007, Free radical biology & medicine.

[18]  T. Matsui,et al.  Inorganic selenium for sheep II. Its influence on rumen bacterial yield, volatile fatty acid production and total tract digestion of timothy hay , 1994 .

[19]  Xu Zi-rong,et al.  Effect of Nano red elemental selenium on GPx activity of broiler chick kidney cellsin vitro , 2003, Wuhan University Journal of Natural Sciences.

[20]  P. V. Soest,et al.  Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. , 1991, Journal of dairy science.

[21]  Kehe Huang,et al.  Effects of different selenium sources on tissue selenium concentrations, blood GSH-Px activities and plasma interleukin levels in finishing lambs , 2007, Biological Trace Element Research.

[22]  F. Dunshea,et al.  Increasing selenium concentration in milk: effects of amount of selenium from yeast and cereal grain supplements. , 2007, Journal of dairy science.

[23]  D. Wilson,et al.  Why are ruminal cellulolytic bacteria unable to digest cellulose at low pH? , 1996, Journal of dairy science.

[24]  J. Doucha,et al.  Production of Chlorella biomass enriched by selenium and its use in animal nutrition: a review , 2009, Applied Microbiology and Biotechnology.

[25]  T. Xu,et al.  Elemental selenium at nano size (Nano-Se) as a potential chemopreventive agent with reduced risk of selenium toxicity: comparison with se-methylselenocysteine in mice. , 2008, Toxicological sciences : an official journal of the Society of Toxicology.

[26]  M. Nazıroğlu,et al.  Effects of vitamin E and selenium on some rumen parameters in lambs. , 1997, Acta Veterinaria Hungarica.

[27]  Sidney A. Williams,et al.  Official Methods of Analysis of the Association of Official Analytical Chemists , 1971, Soil Science Society of America Journal.

[28]  G. Schrauzer.  The nutritional significance, metabolism and toxicology of selenomethionine. , 2003, Advances in food and nutrition research.

[29]  P. Váczi,et al.  Haematological, Blood and Rumen Chemistry Changes in Lambs Following Supplementation with Se-yeast , 2007 .

[30]  R. Phipps,et al.  Effect of dietary supplementation with selenium-enriched yeast or sodium selenite on selenium tissue distribution and meat quality in beef cattle. , 2008, Journal of animal science.

[31]  W. Horwitz.  Official Methods of Analysis , 1980 .

[32]  W. Weiss,et al.  Effect of selenium source on selenium status, neutrophil function, and response to intramammary endotoxin challenge of dairy cows. , 2005, Journal of dairy science.

[33]  W. Yang,et al.  Effects of selenium yeast on rumen fermentation, lactation performance and feed digestibilities in lactating dairy cows , 2009 .