RNA conformational classes.

RNA exhibits a large diversity of conformations. Three thousand nucleotides of 23S and 5S ribosomal RNA from a structure of the large ribosomal subunit were analyzed in order to classify their conformations. Fourier averaging of the six 3D distributions of torsion angles and analyses of the resulting pseudo electron maps, followed by clustering of the preferred combinations of torsion angles were performed on this dataset. Eighteen non-A-type conformations and 14 A-RNA related conformations were discovered and their torsion angles were determined; their Cartesian coordinates are available.

[1]  Wolfram Saenger,et al.  Principles of Nucleic Acid Structure , 1983 .

[2]  C. Vonrhein,et al.  Structure of the 30S ribosomal subunit , 2000, Nature.

[3]  D. McRee Practical Protein Crystallography , 1993 .

[4]  M. D. Newton,et al.  Seven basic conformations of nucleic acid structural units , 1973 .

[5]  T. Steitz,et al.  The complete atomic structure of the large ribosomal subunit at 2.4 A resolution. , 2000, Science.

[6]  W. B. Arendall,et al.  RNA backbone is rotameric , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[7]  G. N. Ramachandran,et al.  Conformation of polypeptides and proteins. , 1968, Advances in protein chemistry.

[8]  H M Berman,et al.  A systematic method for studying the spatial distribution of water molecules around nucleic acid bases. , 1993, Biophysical journal.

[9]  A. Tocilj,et al.  The small ribosomal subunit from Thermus thermophilus at 4.5 A resolution: pattern fittings and the identification of a functional site. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[10]  T. Steitz,et al.  The kink‐turn: a new RNA secondary structure motif , 2001, The EMBO journal.

[11]  C. Kundrot,et al.  RNA Tertiary Structure Mediation by Adenosine Platforms , 1996, Science.

[12]  Emmanuel Tannenbaum,et al.  Automated identification of RNA conformational motifs: theory and application to the HM LSU 23S rRNA. , 2003, Nucleic acids research.

[13]  Harry F. Noller,et al.  The Path of Messenger RNA through the Ribosome , 2001, Cell.

[14]  Jacques H. van Boom,et al.  Molecular structure of a left-handed double helical DNA fragment at atomic resolution , 1979, Nature.

[15]  W. Olson The spatial configuration of ordered polynucleotide chains. I. Helix formation and base stacking , 1976, Biopolymers.

[16]  Anna Marie Pyle,et al.  RNA structure comparison, motif search and discovery using a reduced representation of RNA conformational space. , 2003, Nucleic acids research.

[17]  M. Sundaralingam,et al.  Correlation between the backbone and side chain conformations in 5′‐nucleotides. The concept of a ‘rigid’ nucleotide conformation , 1973 .

[18]  H. Berman,et al.  Geometric Parameters in Nucleic Acids: Sugar and Phosphate Constituents , 1996 .

[19]  N. Seeman,et al.  Crystal structure of a naturally occurring dinucleoside phoaphate: uridylyl 3',5'-adenosine phosphate model for RNA chain folding. , 1972, Journal of molecular biology.

[20]  Frank Harary,et al.  Graph Theory , 2016 .