The nitroblue tetrazolium (NBT) reduction of neutrophil granulocytes from newborn infants and their mothers in relation to the occurrence of bacteria in amnion fluid and cord blood.

The nitroblue tetrazolium (NBT) reduction of granulocytes from cord blood, when measured without in vitro stimulation with bacteria, was significantly higher than that of maternal granulocytes. This elevated activity may be an adequate response to the presence in cord blood of degradation products from the placenta but also of potentially pathogenic bacteria, found in cord blood of infants with high NBT values. On in vitro stimulation cord blood granulocytes displayed the same high NBT activity as those of the mothers and of healthy non-pregnant women. All but one blood culture from the infants 25--48 h after delivery were sterile. The only child with a low non-stimulated NBT activity of cord blood granulocytes was also the only one presenting a clinical picture of neonatal septicemia, though not bacteriologically verified.

[1]  D. Merzbach,et al.  Standardisation of the nitroblue-tetrazolium test. , 1975, Journal of medical microbiology.

[2]  D. Anderson,et al.  Leukocyte function in normal and infected neonates. , 1974, The Journal of pediatrics.

[3]  P. Cocchi,et al.  NITROBLUE‐TETRAZOLIUM REDUCTION BY NEUTROPHILS OF NEWBORN INFANTS IN IN VITRO PHAGOCYTOSIS TEST , 1971, Acta paediatrica Scandinavica.

[4]  M. Fornerod,et al.  Impaired phagocytosis in leukocytes from newborn infants. A study of glycolysis and activities of phosphoglycerate kinase and pyruvate kinase. , 1971, Enzyme.

[5]  R. Good,et al.  Metabolic activities in leukocytes of newborn infants. , 1970, The Journal of pediatrics.

[6]  J. Humbert,et al.  Increased reduction of nitrobule tetrazolium by neutrophils of newborn infants. , 1970, Pediatrics.

[7]  E. Stiehm,et al.  Impaired opsonic activity but normal phagocytosis in low-birth-weight infants. , 1969, The New England journal of medicine.

[8]  R. Coen,et al.  Studies of bactericidal activity and metabolism of the leukocyte in full-term neonates. , 1969, The Journal of pediatrics.

[9]  R. Williams,et al.  Studies on interaction of bacteria, serum factors and polymorphonuclear leukocytes in mothers and newborns. , 1969, Pediatrics.

[10]  M. Miller.  Phagocytosis in the newborn infant: humoral and cellular factors. , 1969, The Journal of pediatrics.

[11]  D. Nathan,et al.  Quantitative nitroblue tetrazolium test in chronic granulomatous disease. , 1968, The New England journal of medicine.

[12]  W. Silverman,et al.  Phagocytosis in premature infants. , 1957, Pediatrics.