Rat brain imaging using full field optical coherence microscopy with short multimode fiber probe

We demonstrated FF OCM(full field optical coherence microscopy) using an ultrathin forward-imaging SMMF (short multimode fiber) probe of 50 μm core diameter, 125 μm diameter, and 7.4 mm length, which is a typical graded-index multimode fiber for optical communications. The axial resolution was measured to be 2.20 μm, which is close to the calculated axial resolution of 2.06 μm. The lateral resolution was evaluated to be 4.38 μm using a test pattern. Assuming that the FWHM of the contrast is the DOF (depth of focus), the DOF of the signal is obtained at 36 μm and that of the OCM is 66 μm. The contrast of the OCT images was 6.1 times higher than that of the signal images due to the coherence gate. After an euthanasia the rat brain was resected and cut at 2.6mm tail from Bregma. Contacting SMMF to the primary somatosensory cortex and the agranular insular cortex of ex vivo brain, OCM images of the brain were measured 100 times with 2μm step. 3D OCM images of the brain were measured, and internal structure information was obtained. The feasibility of an SMMF as an ultrathin forward-imaging probe in full-field OCM has been demonstrated.

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