Metal Nanoparticles/Porous Silicon Microcavity Enhanced Surface Plasmon Resonance Fluorescence for the Detection of DNA

A porous silicon microcavity (PSiMC) with resonant peak wavelength of 635 nm was fabricated by electrochemical etching. Metal nanoparticles (NPs)/PSiMC enhanced fluorescence substrates were prepared by the electrostatic adherence of Au NPs that were distributed in PSiMC. The Au NPs/PSiMC device was used to characterize the target DNA immobilization and hybridization with its complementary DNA sequences marked with Rhodamine red (RRA). Fluorescence enhancement was observed on the Au NPs/PSiMC device substrate; and the minimum detection concentration of DNA ran up to 10 pM. The surface plasmon resonance (SPR) of the MC substrate; which is so well-positioned to improve fluorescence enhancement rather the fluorescence enhancement of the high reflection band of the Bragg reflector; would welcome such a highly sensitive in biosensor.

[1]  J. Storhoff,et al.  A DNA-based method for rationally assembling nanoparticles into macroscopic materials , 1996, Nature.

[2]  N. K. Ali,et al.  Effect of coatings and surface modification on porous silicon nanoparticles for delivery of the anticancer drug tamoxifen , 2016 .

[3]  C. Yoon,et al.  Electrochemical albumin sensing based on silicon nanowires modified by gold nanoparticles , 2011 .

[4]  K. V. Sreekanth,et al.  Graphene–Gold Metasurface Architectures for Ultrasensitive Plasmonic Biosensing , 2015, Advanced materials.

[5]  Claudia Pacholski,et al.  Photonic Crystal Sensors Based on Porous Silicon , 2013, Sensors.

[6]  Haiping He,et al.  Surface plasmon enhanced photoluminescence from porous silicon nanowires decorated with gold nanoparticles , 2016 .

[7]  G. Frens.  Controlled Nucleation for the Regulation of the Particle Size in Monodisperse Gold Suspensions , 1973 .

[8]  X. Lv,et al.  Enhancement of QDs' fluorescence based on porous silicon Bragg mirror , 2015 .

[9]  F. Quignard,et al.  Chitosan-functionalized porous silicon optical transducer for the detection of carboxylic acid-containing drugs in water , 2011 .

[10]  Milan Vala,et al.  Compact surface plasmon-enhanced fluorescence biochip. , 2013, Optics express.

[11]  R. Turan,et al.  Enhanced localized surface plasmon resonance obtained in two step etched silicon nanowires decorated with silver nanoparticles , 2013 .

[12]  Xue-chu Shen,et al.  Localized-surface-plasmon enhanced luminescence from porous silicon by gold nanoparticles , 2010, 2010 3rd International Nanoelectronics Conference (INEC).

[13]  Samuel Achilefu,et al.  Fluorophore–gold nanoparticle complex for sensitive optical biosensing and imaging , 2012, Nanotechnology.

[14]  M. Sailor,et al.  Photoluminescence‐Based Sensing With Porous Silicon Films, Microparticles, and Nanoparticles , 2009 .

[15]  H. Ho,et al.  Nanomaterials enhanced surface plasmon resonance for biological and chemical sensing applications. , 2014, Chemical Society reviews.

[16]  Zhenhong Jia,et al.  High Sensitivity Detection of CdSe/ZnS Quantum Dot-Labeled DNA Based on N-type Porous Silicon Microcavities , 2017, Sensors.

[17]  Seong-Je Park,et al.  Nanosilver Colloids-Filled Photonic Crystal Arrays for Photoluminescence Enhancement , 2010, Nanoscale research letters.

[18]  Lei Jiang,et al.  Hierarchical optical antenna: Gold nanoparticle-modified photonic crystal for highly-sensitive label-free DNA detection , 2012 .

[19]  C. Allender,et al.  Controlling the Nanoscale Patterning of AuNPs on Silicon Surfaces , 2013, Nanomaterials.

[20]  Girija Gaur,et al.  Immobilization of Quantum Dots in Nanostructured Porous Silicon Films: Characterizations and Signal Amplification for Dual‐Mode Optical Biosensing , 2013 .

[21]  Kyujung Kim,et al.  Surface-enhanced plasmon resonance detection of nanoparticle-conjugated DNA hybridization. , 2010, Applied optics.

[22]  Reversible Luminescence Quenching of Porous Si by Solvents , 1991 .

[23]  Leigh T. Canham,et al.  Tuning the Pore Size and Surface Chemistry of Porous Silicon for Immunoassays , 2000 .

[24]  L. Mikac,et al.  Metal Nanoparticles Deposited on Porous Silicon Templates as Novel Substrates for SERS , 2015 .

[25]  Y. Chabal,et al.  Colored porous silicon as support for plasmonic nanoparticles , 2012 .

[26]  Nicolas H Voelcker,et al.  Porous silicon biosensors on the advance. , 2009, Trends in biotechnology.

[27]  B. Rezaei,et al.  Metal (Ni and Bi) coated porous silicon nanostructure, high-performance anode materials for lithium ion batteries with high capacity and stability , 2017 .

[28]  N. Voelcker,et al.  Rapid detection of nicotine from breath using desorption ionisation on porous silicon. , 2017, Chemical communications.

[29]  M. Oueslati,et al.  Towards a biosensor based on anti resonant reflecting optical waveguide fabricated from porous silicon. , 2012, Biosensors & bioelectronics.

[30]  Michael J. Sailor,et al.  Reversible luminescence quenching of porous silicon by solvents , 1992 .

[31]  Zhenhong Jia,et al.  Efficient Fluorescence Resonance Energy Transfer between Quantum Dots and Gold Nanoparticles Based on Porous Silicon Photonic Crystal for DNA Detection , 2017, Sensors.

[32]  Lukas Novotny,et al.  Spectral dependence of single molecule fluorescence enhancement. , 2007, Optics express.

[33]  V. Levi,et al.  Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles. , 2012, The journal of physical chemistry. B.

[34]  E. Pérez,et al.  Biosensing and protein fluorescence enhancement by functionalized porous silicon devices. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[35]  Robert Romestain,et al.  Improvement of the luminescence in p-type as-prepared or dye impregnated porous silicon microcavities , 1998 .