Efficacy of associative N2-fixation by streptomycin-resistant mutants of Azospirillum brasilense with genotypes of chick pea Rhizobium strains

SUMMARY Azospirillum brasilense was treated with nitrosoguanidine and two mutants isolated which were resistant to 500 μg streptomycin/ml. Ultraviolet sensitivity, photoreactivation and effect of acriflavin on pre- and post-irradiation were studied. Chick pea showed an increase in grain yield, nodule dry weight, N2ase activity and active iron content of nodules when inoculated with A. brasilense or its mutants together with Rhizobium. The interaction between Rhizobium strains and genotypes of chick pea was significant.