Photoionization of a protein isolated in vacuo.

Gas phase VUV single-photon photoionization spectroscopy of electrospray-produced multiply protonated cytochrome c protein (12 kDa) has been performed by means of coupling a linear quadrupole ion trap with a synchrotron beamline. The thresholds for the ionization of the 8+ and 11+ charge state precursors to radical 9+ and 12+ species were measured to be 12.75 ± 0.10 and 13.51 ± 0.10 eV, respectively.

[1]  S. Bari,et al.  Photodissociation of protonated leucine-enkephalin in the VUV range of 8-40 eV. , 2011, The Journal of chemical physics.

[2]  P. Mach,et al.  Electron impact ionization of furanose alcohols. , 2010, The Journal of chemical physics.

[3]  R. Zubarev,et al.  Electron ionization dissociation of singly and multiply charged peptides. , 2009, Journal of the American Chemical Society.

[4]  A. D. Jones,et al.  Femtosecond laser-induced ionization/dissociation of protonated peptides. , 2009, Journal of the American Chemical Society.

[5]  P. Dugourd,et al.  Electron photodetachment of trapped doubly deprotonated angiotensin peptides. UV spectroscopy and radical recombination , 2009 .

[6]  M. Coreno,et al.  Photoionization cross section of Xe+ ion in the pure 5p5 2P3/2 ground level. , 2008, Physical review letters.

[7]  Yves Gimbert,et al.  Internal energy distribution of peptides in electrospray ionization : ESI and collision-induced dissociation spectra calculation. , 2008, Journal of mass spectrometry : JMS.

[8]  P. Dugourd,et al.  Electron photodetachment from gas phase peptide dianions. Relation with optical absorption properties. , 2008, The journal of physical chemistry. A.

[9]  P. Dugourd,et al.  Ultraviolet spectroscopy of peptide and protein polyanions in vacuo: signature of the ionization state of tyrosine. , 2007, Journal of the American Chemical Society.

[10]  Laurent Nahon,et al.  Design, Construction and Magnetic Measurements of the HU640 (OPHELIE2) Undulator dedicated to the DESIRS VUV Beamline at SOLEIL , 2007 .

[11]  S. Leone,et al.  Direct determination of the ionization energy of histidine with VUV synchrotron radiation , 2006 .

[12]  E. De Pauw,et al.  Internal energy and fragmentation of ions produced in electrospray sources. , 2005, Mass spectrometry reviews.

[13]  Jos Oomens,et al.  Charge-state resolved mid-infrared spectroscopy of a gas-phase protein. , 2005, Physical chemistry chemical physics : PCCP.

[14]  H. Schwarz,et al.  Revision of the second ionization energy of toluene. , 2005, The Journal of chemical physics.

[15]  K. Fuke,et al.  Structures and reactions of biomolecular ions produced with electrospray ionization , 2003 .

[16]  A. Simon,et al.  Gas phase infrared spectroscopy of selectively prepared ions. , 2002, Physical review letters.

[17]  B. Budnik,et al.  Ionization energies of multiply protonated polypeptides obtained by tandem ionization in Fourier transform mass spectrometers. , 2002, Journal of mass spectrometry : JMS.

[18]  F. Folkmann,et al.  Measurements of the absolute photoionization cross section of Fe+ ions from 15.8 to 180 eV , 2002 .

[19]  B. Lagarde,et al.  Very high spectral resolution obtained with SU5: A vacuum ultraviolet undulator-based beamline at Super-ACO , 2001 .

[20]  O. Dutuit,et al.  Experimental and theoretical study of a differentially pumped absorption gas cell used as a low energy-pass filter in the vacuum ultraviolet photon energy range , 2000 .

[21]  J. Berkowitz.  The Quantum Yield of Ionization , 2000 .

[22]  C. Hendrickson,et al.  Unequivocal determination of metal atom oxidation state in naked heme proteins: Fe(III)myoglobin, Fe(III)cytochrome c, Fe(III)cytochrome b5, and Fe(III)cytochrome b5 L47R , 2000, Journal of the American Society for Mass Spectrometry.

[23]  B. Budnik,et al.  MH2+⋅ ion production from protonated polypeptides by electron impact: observation and determination of ionization energies and a cross-section , 2000 .

[24]  Drahos,et al.  Thermal energy distribution observed in electrospray ionization , 1999, Journal of mass spectrometry : JMS.

[25]  E. Pauw,et al.  Calibration of the Internal Energy Distribution of Ions Produced by Electrospray , 1998 .

[26]  M. Jarrold,et al.  PROTEIN STRUCTURE IN VACUO : GAS-PHASE CONFORMATIONS OF BPTI AND CYTOCHROME C , 1997 .

[27]  M. Thoss,et al.  Model study of near-threshold photoionization of large molecules: The effect of vibrational relaxation , 1997 .

[28]  R. Levine,et al.  Do large molecules ionize , 1992 .

[29]  J. West,et al.  Measurements of absolute cross sections for the photoionisation of Ba+ ions , 1986 .

[30]  H. Inokuchi,et al.  Ionization potentials of ferricytochrome c, ferrocytochrome c, and ferricytochrome c3 , 1978 .

[31]  G. Wannier.  The Threshold Law for Single Ionization of Atoms or Ions by Electrons , 1953 .