Lens-Coupled CCD Detector for X-ray Crystallography

An X-ray crystallography detector (Blue-1) has been built based upon a Fairchild 486 back-illuminated CCD and a custom lens system designed by Optics One Inc. The advantages of our Blue-1 lens system over more conventional fiber-optic tapers are: lower noise and higher efficiency; improved point spread function; negligible spatial distortion; and lack of "chicken-wire" patterns. Also, the engineering is simpler because the CCD is not bonded to the fiber-optic taper. A unique mechanical design has been employed to accurately focus the image on the CCD. The detector software is based on MATLAB and takes advantage of its powerful imaging and signal processing libraries. The CCD timing can be updated on the fly by using a "CCD controller language" to specify timing.

[1]  Clif Flynt Tcl/Tk For Real Programmers , 1998 .

[2]  D. O'Mara,et al.  Area detector design Part II. Application to a modular CCD-based detector for X-ray crystallography , 1993 .

[3]  D. O'Mara,et al.  Characterization and data collection on a direct-coupled CCD X-ray detector , 1994 .

[4]  George Zentai,et al.  Charge-coupled device/fiber optic taper array x-ray detector for protein crystallography , 1997 .

[5]  Walter Charles Phillips,et al.  The detective quantum efficiency of CCD and vidicon-based detectors for X-ray crystallographic applications , 1992 .

[6]  Paul Vu,et al.  Design and characterization of a large-area lens-coupled CCD detector for macromolecular crystallography , 2003, SPIE Optics + Photonics.

[7]  D.F. Barbe,et al.  Imaging devices using the charge-coupled concept , 1975, Proceedings of the IEEE.

[8]  John Harris,et al.  Handbook of mathematics and computational science , 1998 .

[9]  Sol M. Grunera Charge-coupled device area x-ray detectors , 2002 .

[10]  Istvan Naday,et al.  [17] Charge-coupled device-based area detectors. , 1997, Methods in enzymology.

[11]  M. Stanton Area detector design Part I. Formulation of design criteria and application to X-ray crystallography , 1993 .

[12]  The technology that enables synchrotron structural biology , 1998, Nature Structural Biology.

[13]  M. R. Kraimer,et al.  CCD(charge-coupled device)-based synchrotron x-ray detector for protein crystallography: Performance projected from an experiment , 1986 .

[14]  John R. Helliwell,et al.  Macromolecular Crystallography with Synchrotron Radiation , 1992 .

[15]  Daniel M. O'Mara,et al.  Modular CCD detector for x-ray crystallography , 1993, Electronic Imaging.

[16]  Wayne A Hendrickson,et al.  [28] Phase determination from multiwavelength anomalous diffraction measurements. , 1997, Methods in enzymology.

[17]  Youli Li,et al.  Correcting spatial distortions and nonuniform response in area detectors , 1992 .

[18]  M. Kraimer,et al.  Experimental Physics and Industrial Control System (EPICS): Application source/release control for EPICS R3.11.6 , 1994 .

[19]  L. Mortara,et al.  Evaluations Of Charge-Coupled Device (CCD) Performance For Astronomical Use , 1981, Other Conferences.

[20]  C. I. Coleman Imaging Characteristics of Rigid Coherent Fiber Optic Tapers , 1985 .

[21]  E M Westbrook,et al.  Charge-coupled device-based area detectors. , 1997, Methods in enzymology.

[22]  W. Hendrickson Determination of macromolecular structures from anomalous diffraction of synchrotron radiation. , 1991, Science.

[23]  Robert M. Sweet,et al.  Ccd-Based Detector for Protein Crystallography with Synchrotron X-Rays , 1990 .

[24]  K. Holmes From rare to routine , 1998, Nature Structural Biology.

[25]  David J. Thomas Calibrating an area-detector diffractometer: integral response , 1990, Proceedings of the Royal Society of London. A. Mathematical and Physical Sciences.