Tunable filter-based multispectral imaging for detection of blood stains on construction material substrates part 2: realization of rapid blood stain detection.

Based on the blood stain detection method and criteria established in part 1 of this article, we combine and organize all necessary tasks to realize the multispectral imaging-based rapid blood stain detection system. To rapidly detect blood stains on the test surface, the developed system automatically captures the spectral images, extracts their spectral data, determines the positions of blood stains, and accurately highlights the positions of blood stains on the display. To achieve such a system, several tasks are newly introduced, including adjustment of camera exposure times to prevent image saturation or excessive darkness, the search for the sampled clean positions of the substrate to determine the substrate reflectance spectrum, and suitable detection procedures and proper arrangement of criteria to eliminate unnecessary calculations. Parallel processes between image capturing and blood stain identification help shorten the time for blood stain identifications despite a large amount of spectral data to be processed. The developed system can identify blood against several other reddish brown stains on several substrates. The measured average identification times on different test surfaces range from only 23.3 to 28.7 s, including the image capturing process.

[1]  John Harry Wagner,et al.  Applications of UV-visible spectral imaging in forensic science , 2008 .

[2]  Multimode imaging in the thermal infrared for chemical contrast enhancement. Part 2: Simulation driven design. , 2010, Analytical chemistry.

[3]  Multimode imaging in the thermal infrared for chemical contrast enhancement. Part 1: Methodology. , 2010, Analytical chemistry.

[4]  Multimode imaging in the thermal infrared for chemical contrast enhancement. Part 3: Visualizing blood on fabrics. , 2010, Analytical chemistry.

[5]  A. C. Ponce,et al.  Critical Revision of Presumptive Tests for Bloodstains , 1999 .

[6]  Annemarie Nadort,et al.  Age estimation of blood stains by hemoglobin derivative determination using reflectance spectroscopy. , 2011, Forensic science international.

[7]  B. Khoo,et al.  Forensic Light Sources for Detection of Biological Evidences in Crime Scene Investigation: A Review , 2010 .

[8]  G. Miskelly,et al.  Using spectral information in forensic imaging. , 2005, Forensic science international.

[9]  S. Sumriddetchkajorn,et al.  Tunable filter-based multispectral imaging for detection of blood stains on construction material substrates. Part 1. Developing blood stain discrimination criteria. , 2012, Applied optics.

[10]  R. V. Oorschot,et al.  The Use of Polilight® in the Detection of Seminal Fluid, Saliva, and Bloodstains and Comparison with Conventional Chemical‐Based Screening Tests , 2006, Journal of forensic sciences.

[11]  M. Cox,et al.  A study of the sensitivity and specificity of four presumptive tests for blood. , 1991, Journal of forensic sciences.

[12]  A. Gross,et al.  The effect of luminol on presumptive tests and DNA analysis using the polymerase chain reaction. , 1999, Journal of forensic sciences.

[13]  Li-Chin Tsai,et al.  Forensic Applications of Infrared Imaging for the Detection and Recording of Latent Evidence , 2007, Journal of forensic sciences.