ApoE enhances nanodisk-mediated curcumin delivery to glioblastoma multiforme cells.

AIM To evaluate the effect of incorporating the polyphenol, curcumin, into nanodisk (ND) particles on its biological activity. MATERIALS & METHODS Curcumin-NDs formulated with different scaffold proteins were incubated with cultured glioblastoma multiforme cells. RESULTS When ApoE was employed as the ND scaffold protein, enhanced curcumin uptake was observed. Furthermore, ApoE curcumin-NDs induced greater cell death than either free curcumin or ApoAI curcumin-NDs. A total of 1 h after exposure of glioblastoma multiforme cells to ApoE curcumin-NDs, significant curcumin uptake was detected while ApoE was localized at the cell surface. After 2 h, a portion of the curcumin had migrated to the nucleus, giving rise to enhanced fluorescence intensity in discrete intranuclear sites. CONCLUSION ApoE-mediated interaction of curcumin-NDs with glioblastoma multiforme cells leads to enhanced curcumin uptake and increased biological activity.

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