Recovery and quantitative detection of thiabendazole on apples using a surface swab capture method followed by surface-enhanced Raman spectroscopy.

We developed a rapid and simple method which combines a surface swab capture method and surface-enhanced Raman spectroscopy for recovery and quantitative detection of thiabendazole on apple surfaces. The whole apple surface was swabbed and the swab was vortexed in methanol releasing the pesticide. Silver dendrites were then added to bind the pesticide and used for enhancing the Raman signals. The recovery of the surface swab method was calculated to be 59.4-76.6% for intentionally contaminated apples at different levels (0.1, 0.3, 3, and 5 ppm, μg/g per weight). After considering the releasing factor (66.6%) from the swab, the final accuracy of the swab-SERS method was calculated to be between 89.2% and 115.4%. This swab-SERS method is simple, sensitive, rapid (∼10 min), and quantitative enough for QA/QC in plant procedure. This can be extended to detect other pesticides on raw agricultural produce like pears, carrots, and melons etc.

[1]  Eun Kyu Lee,et al.  Quantitative Analysis of Methyl Parathion Pesticides in a Polydimethylsiloxane Microfluidic Channel Using Confocal Surface-Enhanced Raman Spectroscopy , 2006, Applied spectroscopy.

[2]  Lili He,et al.  Rapid detection of ricin in milk using immunomagnetic separation combined with surface-enhanced Raman spectroscopy. , 2011, Journal of food science.

[3]  U. Meyer,et al.  Comparison of prevalence of microorganisms on titanium and silicone/polymethyl methacrylate obturators used for rehabilitation of maxillary defects. , 2008, The Journal of prosthetic dentistry.

[4]  Theodore P. Labuza,et al.  Rapid detection of a foreign protein in milk using IMS-SERS , 2011 .

[5]  Miguel Valcárcel,et al.  Determination of pesticides by capillary chromatography and SERS detection using a novel Silver-Quantum dots "sponge" nanocomposite. , 2012, Journal of Chromatography A.

[6]  J. Popp,et al.  Surface-enhanced Raman spectroscopy , 2009, Analytical and bioanalytical chemistry.

[7]  Theodore P. Labuza,et al.  Aptamer-based surface-enhanced Raman scattering detection of ricin in liquid foods , 2011 .

[8]  Nam-Jung Kim,et al.  Surface‐enhanced Raman spectroscopy coupled with dendritic silver nanosubstrate for detection of restricted antibiotics , 2009 .

[9]  C. A. Davidson,et al.  Evaluation of two methods for monitoring surface cleanliness-ATP bioluminescence and traditional hygiene swabbing. , 1999, Luminescence : the journal of biological and chemical luminescence.

[10]  D. McNaughton,et al.  Surface-enhanced Raman spectroscopic analysis of fonofos pesticide adsorbed on silver and gold nanoparticles , 2010 .

[11]  Z. Liron,et al.  Surface-enhanced Raman scattering detection of cholinesterase inhibitors. , 2011, Analytica chimica acta.

[12]  C. Haynes,et al.  Detection of a foreign protein in milk using surface-enhanced Raman spectroscopy coupled with antibody-modified silver dendrites. , 2011, Analytical chemistry.

[13]  L. Rossi,et al.  High performance gold nanorods and silver nanocubes in surface-enhanced Raman spectroscopy of pesticides. , 2009, Physical chemistry chemical physics : PCCP.

[14]  Richard L. McCreery,et al.  Raman Spectroscopy for Chemical Analysis , 2000 .

[15]  Qingqing Li,et al.  Determination of tricyclazole content in paddy rice by surface enhanced Raman spectroscopy. , 2012, Journal of food science.

[16]  Rafał Lewandowski,et al.  Use of a Foam Spatula for Sampling Surfaces after Bioaerosol Deposition , 2009, Applied and Environmental Microbiology.

[17]  Min Kyung Kim,et al.  Surface-enhanced Raman Spectroscopy of Benzimidazolic Fungicides: Benzimidazole and Thiabendazole , 2009 .

[18]  B. Liu,et al.  Detection of Pesticides in Fruits by Surface-Enhanced Raman Spectroscopy Coupled with Gold Nanostructures , 2013, Food and Bioprocess Technology.