Properties of Q -replicase protein synthesized in a cell-free system.

The largest polypeptide obtained as a product of Qβ RNA-stimulated cell-free protein synthesis is the replicase (R) protein. The R polypeptide synthesized in vitro has a chain length of approximately 550 residues and is identical in size to the β chain of purified replicase. From its electrophoretic behavior at pH 8.5, a net charge of -27 and a cysteine content of > 15 residues can be deduced. A portion of R polypeptide synthesized in vitro binds to added Qβ RNA and this binding is more resistant to increased ionic strength than the binding of the positively charged coat protein to Qβ RNA. Another portion of the R polypeptide binds to some constituent of the Escherichia coli extract to form a 20-S complex.

[1]  H. Lodish,et al.  Removal of formyl-methionine residue from nascent bacteriophage f2 protein. , 1972, Journal of molecular biology.

[2]  R. E. Webster,et al.  Gene products of bacteriophage Qβ , 1971 .

[3]  W. Zillig,et al.  Reconstitution of bacterial DNA‐dependent RNA‐polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription , 1970, FEBS letters.

[4]  C. Weissmann,et al.  Subunit Structure of Qβ Replicase , 1970, Nature.

[5]  R. Kamen.  Characterization of the Subunits of Qβ Replicase , 1970, Nature.

[6]  P. Kaesberg,et al.  Synthesis of polypeptides directed by the RNA of phage Q beta. , 1970, Virology.

[7]  K. Weber,et al.  Amino-acid Sequence of the Qβ Coat Protein , 1970, Nature.

[8]  M. E. Reichmann,et al.  A mechanism of the irreversible inactivation of bovine pancreatic ribonuclease by diethylpyrocarbonate. A general reaction of diethylpyrocarbonate . A general reaction of diethylpyrocarbonate with proteins. , 1970, European journal of biochemistry.

[9]  K. Weber,et al.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. , 1969, The Journal of biological chemistry.

[10]  A. Sillero,et al.  Virus-specific proteins in Escherichia coli infected with phage Qb. , 1969, Biochimica et biophysica acta.

[11]  G. Apell,et al.  The amino acid sequence of bovine liver catalase: a preliminary report. , 1969, Archives of biochemistry and biophysics.

[12]  M. Capecchi,et al.  Cell-free protein synthesis programmed with R17 RNA: identification of two phage proteins. , 1966, Journal of molecular biology.

[13]  J. H. Strauss,et al.  Purification and properties of bacteriophage MS2 and of its ribonucleic acid. , 1963, Journal of molecular biology.

[14]  A. Tissières,et al.  Ribonucleoprotein Particles from Escherichia Coli , 1958, Nature.

[15]  L. Shapiro,et al.  Synthesis of bacteriophage Q-beta RNA. , 1968, Cold Spring Harbor Symposia on Quantitative Biology.

[16]  T. Aronsson,et al.  Improved separation of serum proteins in paper electrophoresis: a new electrophoresis buffer. , 1957, Scandinavian journal of clinical and laboratory investigation.