论文信息 - High resolution 3D imaging of primary and secondary tumor spheroids using multicolor multi-angle Light Sheet Fluorescence Microscopy (LSFM)

High resolution 3D imaging of primary and secondary tumor spheroids using multicolor multi-angle Light Sheet Fluorescence Microscopy (LSFM)

Breast cancer and Glioblastoma brain cancer are aggressive malignancies with poor prognosis. In this study primary Glioblastoma and secondary breast cancer spheroids are formed and treated with the well-known Temozolomide and Doxorubicin chemotherapeutics, respectively. A custom multi-angle Light Sheet Fluorescence Microscope is employed for high resolution imaging of both cancer cell spheroids. Such a technique is successful in realizing pre-clinical drug screening, while enables the discrimination among physiologic tumor parameters. LSFM technique, parameters and method followed are also presented.

[1] Lars Hufnagel,et al. Multiview light-sheet microscope for rapid in toto imaging , 2012, Nature Methods.

[2] Uros Krzic,et al. Light sheet‐based fluorescence microscopy: More dimensions, more photons, and less photodamage , 2008, HFSP journal.

[3] F. Del Bene,et al. Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy , 2004, Science.

[4] Ernst H. K. Stelzer,et al. High-resolution deep imaging of live cellular spheroids with light-sheet-based fluorescence microscopy , 2013, Cell and Tissue Research.

[5] Jorge Ripoll,et al. A Customized Light Sheet Microscope to Measure Spatio-Temporal Protein Dynamics in Small Model Organisms , 2015, PloS one.

[6] Eleftheria Tzamali,et al. A hybrid discrete-continuous model of in vitro spheroid tumor growth and drug response , 2016, 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC).

[7] V Sakkalis,et al. In Vitro/In Silico Study on the Role of Doubling Time Heterogeneity among Primary Glioblastoma Cell Lines , 2017, BioMed research international.