Photothermal therapy using gold nanorods and near-infrared light in a murine melanoma model increases survival and decreases tumor volume

Photothermal therapy (PTT) treatments have shown strong potential in treating tumors through their ability to target destructive heat preferentially to tumor regions. In this paper we demonstrate that PTT in a murine melanoma model using gold nanorods (GNRs) and near-infrared (NIR) light decreases tumor volume and increases animal survival to an extent that is comparable to the current generation of melanoma drugs. GNRs, in particular, have shown a strong ability to reach ablative temperatures quickly in tumors when exposed to NIR light. The current research tests the efficacy of GNRs PTT in a difficult and fast growing murine melanoma model using a NIR light-emitting diode (LED) light source. LED light sources in the NIR spectrum could provide a safer and more practical approach to photothermal therapy than lasers. We also show that the LED light source can effectively and quickly heat in vitro and in vivo models to ablative temperatures when combined with GNRs. We anticipate that this approach could have significant implications for human cancer therapy.

[1]  Khaled Greish,et al.  Enhanced permeability and retention (EPR) effect for anticancer nanomedicine drug targeting. , 2010, Methods in molecular biology.

[2]  Wei Zhang,et al.  Thermal ablation versus conventional regional hyperthermia has greater anti-tumor activity against melanoma in mice by upregulating CD4 + cells and enhancing IL-2 secretion , 2009 .

[3]  I. Fidler,et al.  Selection of successive tumour lines for metastasis. , 1973, Nature: New biology.

[4]  Lawrence Tamarkin,et al.  Colloidal Gold: A Novel Nanoparticle Vector for Tumor Directed Drug Delivery , 2004, Drug delivery.

[5]  Wei Lu,et al.  Effects of photoacoustic imaging and photothermal ablation therapy mediated by targeted hollow gold nanospheres in an orthotopic mouse xenograft model of glioma. , 2011, Cancer research.

[6]  T. Niidome,et al.  The effects of PEG grafting level and injection dose on gold nanorod biodistribution in the tumor-bearing mice. , 2009, Journal of controlled release : official journal of the Controlled Release Society.

[7]  J. Schachter,et al.  Immunotherapy for the Management of Advanced Melanoma: The Next Steps , 2013, American Journal of Clinical Dermatology.

[8]  S. Bhatia,et al.  GOLD NANOROD PHOTOTHERMAL THERAPY IN A GENETICALLY ENGINEERED MOUSE MODEL OF SOFT TISSUE SARCOMA , 2010 .

[9]  C. Allen,et al.  Block copolymer micelles for delivery of cancer therapy: transport at the whole body, tissue and cellular levels. , 2009, Journal of controlled release : official journal of the Controlled Release Society.

[10]  Glenn P. Goodrich,et al.  Photothermal Efficiencies of Nanoshells and Nanorods for Clinical Therapeutic Applications , 2009 .

[11]  Ji-Xin Cheng,et al.  Gold Nanorods as Contrast Agents for Biological Imaging: Optical Properties, Surface Conjugation and Photothermal Effects † , 2009, Photochemistry and photobiology.

[12]  Wei Lu,et al.  Targeted Photothermal Ablation of Murine Melanomas with Melanocyte-Stimulating Hormone Analog–Conjugated Hollow Gold Nanospheres , 2009, Clinical Cancer Research.

[13]  Michael J Sailor,et al.  Computationally guided photothermal tumor therapy using long-circulating gold nanorod antennas. , 2009, Cancer research.

[14]  Ji-Xin Cheng,et al.  Hyperthermic effects of gold nanorods on tumor cells. , 2007, Nanomedicine.

[15]  Esther H Chang,et al.  Does a targeting ligand influence nanoparticle tumor localization or uptake? , 2008, Trends in biotechnology.

[16]  J. M. Harris,et al.  Pegylation: a novel process for modifying pharmacokinetics. , 2001, Clinical pharmacokinetics.

[17]  Shahram Hejazi,et al.  Review of Long-Wavelength Optical and NIR Imaging Materials: Contrast Agents, Fluorophores and Multifunctional Nano Carriers. , 2012, Chemistry of materials : a publication of the American Chemical Society.

[18]  Hao Hong,et al.  Applications of gold nanoparticles in cancer nanotechnology. , 2008, Nanotechnology, science and applications.

[19]  M. Dewhirst,et al.  Thresholds for thermal damage to normal tissues: An update , 2011, International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group.

[20]  H. Maeda,et al.  Tumor vascular permeability and the EPR effect in macromolecular therapeutics: a review. , 2000, Journal of controlled release : official journal of the Controlled Release Society.

[21]  Daniel Day,et al.  Cancer cell imaging and photothermal therapy using gold nanorods , 2008 .

[22]  Chee-Youb Won,et al.  PEG-modified biopharmaceuticals. , 2009, Expert opinion on drug delivery.

[23]  Michael J Sailor,et al.  SERS‐Coded Gold Nanorods as a Multifunctional Platform for Densely Multiplexed Near‐Infrared Imaging and Photothermal Heating , 2009, Advanced materials.

[24]  Manuela Semmler-Behnke,et al.  Biodistribution of PEG-modified gold nanoparticles following intratracheal instillation and intravenous injection. , 2010, Biomaterials.

[25]  Nastassja A. Lewinski,et al.  A new era for cancer treatment: gold-nanoparticle-mediated thermal therapies. , 2011, Small.

[26]  M. Olivo,et al.  Critical parameters in the pegylation of gold nanoshells for biomedical applications: An in vitro macrophage study , 2009, Journal of drug targeting.

[27]  Prashant K. Jain,et al.  Plasmonic photothermal therapy (PPTT) using gold nanoparticles , 2008, Lasers in Medical Science.

[28]  R. Mahato,et al.  Extravasation of polymeric nanomedicines across tumor vasculature. , 2011, Advanced drug delivery reviews.

[29]  Richard Su,et al.  Highly purified biocompatible gold nanorods for contrasted optoacoustic imaging of small animal models. , 2012, Nanoscience and nanotechnology letters.

[30]  Erik C. Dreaden,et al.  Gold nanorod assisted near-infrared plasmonic photothermal therapy (PPTT) of squamous cell carcinoma in mice. , 2008, Cancer letters.

[31]  K. McMasters,et al.  Current management of melanoma. , 2013, Current problems in surgery.

[32]  P. Jain,et al.  Calculated absorption and scattering properties of gold nanoparticles of different size, shape, and composition: applications in biological imaging and biomedicine. , 2006, The journal of physical chemistry. B.

[33]  A. Ray,et al.  Guided Delivery of Polymer Therapeutics Using Plasmonic Photothermal Therapy. , 2012, Nano today.

[34]  C. Chiang,et al.  First demonstration of gold nanorods-mediated photodynamic therapeutic destruction of tumors via near infra-red light activation. , 2014, Small.

[35]  C. Robert,et al.  New drugs in melanoma: it's a whole new world. , 2011, European journal of cancer.

[36]  Dominique Barchiesi,et al.  Quantitative comparison of optimized nanorods, nanoshells and hollow nanospheres for photothermal therapy , 2012, Biomedical optics express.