Cerebellar nuclei and the nucleocortical projections in the rat: Retrograde tracing coupled to GABA and glutamate immunohistochemistry

The amino acids GABA and glutamate (Glu) are thought to be the principal substances in the central nervous system responsible for neuronal inhibition and excitation. Their distributions among the different neurons in a defined pathway may thus be indicative of the contributions of the cells to pathway function. Examples of such neurons are those of the cerebellar nuclei which, while regulating output from the Purkinje cells of the cerebellar cortex, are also found to project back to the cerebellar cortex.

[1]  P. Somogyi,et al.  Quantification of immunogold labelling reveals enrichment of glutamate in mossy and parallel fibre terminals in cat cerebellum , 1986, Neuroscience.

[2]  V. Chan‐Palay,et al.  The Cerebellar Dentate Nucleus , 1977 .

[3]  T. Kuno,et al.  Immunohistochemical localization of γ-aminobutyric acid- and aspartate-containing neurons in the rat deep cerebellar nuclei , 1988, Brain Research.

[4]  M. Cuénod,et al.  Afferents to the rat red nucleus studied by means ofd-[3H] aspart ate, [3H]choline and non-selective tracers , 1988, Neuroscience.

[5]  W. Oertel,et al.  Immunocytochemical localization of glutamate decar☐ylase in rat cerebellum with a new antiserum , 1981, Neuroscience.

[6]  H. Bantli,et al.  Anatomical and physiological evidence for a cerebellar nucleo-cortical projection in the cat , 1976, Neuroscience.

[7]  C. Batini,et al.  Brain stem nuclei giving fibers to lobules VI and VII of the cerebellar vermis , 1978, Brain Research.

[8]  D. Tolbert,et al.  Uptake and transport of 3H-GABA (γ-aminobutyric acid) injected into the cat dentate nucleus , 1980, Experimental Neurology.

[9]  Takehiko Umetani,et al.  Topographic organization of the cerebellar nucleocortical projection in the albino rat: an autoradiographic orthograde study , 1990, Brain Research.

[10]  Ann M. Graybiel,et al.  Afferents to the cerebellar cortex in the cat: evidence for an intrinsic pathway leading from the deep nuclei to the cortex , 1976, Brain Research.

[11]  R. Semba,et al.  Purification of specific antibody against aspartate and immunocytochemical localization of aspartergic neurons in the rat brain , 1987, Neuroscience.

[12]  D. T. Hess.  Cerebellar nucleo-cortical neurons projecting to the vermis of lobule VII in the rat , 1982, Brain Research.

[13]  J C Pearson,et al.  Cerebellar corticonuclear – nucleocortical topography: A study of the tree shrew (Tupaia) paraflocculus , 1979, The Journal of comparative neurology.

[14]  A. Nieoullon,et al.  Decrease in choline acetyltransferase and in high affinity glutamate uptake in the red nucleus of the cat after cerebellar lesions , 1981, Neuroscience Letters.

[15]  P. Angaut,et al.  Anatomical mapping of the cerebellar nucleocortical projections in the rat: A retrograde labeling study , 1989, The Journal of comparative neurology.

[16]  G. Bishop,et al.  Morphological and electrophysiological characteristics of projection neurons in the nucleus interpositus of the cat cerebellum , 1978, The Journal of comparative neurology.

[17]  V. Chan‐Palay.  Neurotransmitters and Receptors in the Cerebellum: Immunocytochemical Localization of Glutamic Acid Decarboxylase, GABA-Transaminase, and Cyclic GMP and Autoradiography with 3H-Muscimol , 1982 .

[18]  A I Basbaum,et al.  Wheat germ agglutinin‐apoHRP gold: A new retrograde tracer for light‐ and electron‐microscopic single‐ and double‐label studies , 1987, The Journal of comparative neurology.

[19]  N. Dusticier,et al.  Decrease in choline acetyltransferase activity in the red nucleus of the cat after cerebellar lesion , 1981, Neuroscience.

[20]  T. Kaneko,et al.  Immunohistochemical study of glutaminase‐containing neurons in the cerebral cortex and thalamus of the rat , 1988, The Journal of comparative neurology.

[21]  A. Beitz,et al.  Monoclonal antibodies specific for fixative-modified aspartate: immunocytochemical localization in the rat CNS , 1987, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[22]  F. Walberg,et al.  Aspartate-like and glutamate-like immunoreactivities in the inferior olive and climbing fibre system: A light microscopic and semiquantitative electron microscopic study in rat and baboon (Papio anubis) , 1990, Neuroscience.

[23]  S. Hsu,et al.  Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. , 1981, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[24]  J. Storm-Mathisen,et al.  Glutamate‐ and GABA‐containing neurons in the mouse and rat brain, as demonstrated with a new immunocytochemical technique , 1984, The Journal of comparative neurology.

[25]  P. Somogyi,et al.  Antisera to gamma-aminobutyric acid. I. Production and characterization using a new model system. , 1985, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[26]  C. Batini,et al.  The GABAergic neurones of the cerebellar nuclei in the rat: Projections to the cerebellar cortex , 1989, Neuroscience Letters.

[27]  B. Newton,et al.  Immunohistochemistry of γ-aminobutyric acid in the cat nucleus tractus solitarius , 1985, Brain Research.

[28]  E. Mugnaini,et al.  Origins of GABAergic Inputs to the Inferior Olive , 1989 .

[29]  V. Gallo,et al.  Expression of Excitatory Amino Acid Receptors by Cerebellar Cells of the Type‐2 Astrocyte Cell Lineage , 1989, Journal of neurochemistry.

[30]  M. Stewart,et al.  GABA‐immunoreactive neurons in the rat cerebellum: A light and electron microscope study , 1986, The Journal of comparative neurology.

[31]  Masao Ito.  The Cerebellum And Neural Control , 1984 .

[32]  A. Brodal,et al.  The Inferior Olive. Notes on its Comparative Anatomy, Morphology, and Cytology , 1980 .

[33]  J. N. Payne.  The cerebellar nucleo-cortical projection in the rat studied by the retrograde fluorescent double-labelling method , 1983, Brain Research.

[34]  T. Kaneko,et al.  Glutaminase-like immunoreactivity in the lower brainstem and cerebellum of the adult rat , 1989, Neuroscience.

[35]  A. Beitz,et al.  An ultrastructural description of glutamate-like immunoreactivity in the rat cerebellar cortex , 1987, Brain Research.

[36]  C. Sotelo,et al.  Synaptology of the cerebello-olivary pathway. Double labelling with anterograde axonal tracing and GABA immunocytochemistry in the rat , 1989, Brain Research.

[37]  A. Beitz,et al.  Immunocytochemical localization of glutamate-, glutaminase- and aspartate aminotransferase-like immunoreactivity in the rat deep cerebellar nuclei , 1986, Brain Research.