Experimental and simulation analysis of membrane adsorbers used for the primary capture step in antibody manufacturing

Membrane chromatography is a novel protein purification technique developed to overcome the major limitations due to packed beads, such as long process time, mass transport controlled by diffusion and high pressure drops. In this work, the adsorption of human IgG onto new Protein A affinity membranes has been studied in detail. To this aim, several chromatographic cycles have been measured at different experimental conditions. A mathematical model for protein purification with affinity membrane adsorbers has been developed and validated with experimental data.