The chick chorioallantoic membrane as a novel in vivo model for the testing of biomaterials.

Current in vivo models for testing biomaterials are time and labor intensive as well as expensive. This article describes a new approach for testing biomaterials in vivo using the chorioallantoic membrane (CAM) of the developing chicken embryo, as an alternative to the traditional mammalian models. Fertilized chicken eggs were incubated for 4 days, at which time a small window was cut in the shell of the egg. After 1 week of incubation, the CAM received several test materials, including the endotoxin LPS, a cotton thread and a Silastic tubing. One day and 1 week later, the tissue response to the test materials was assessed using gross, histological, and scanning electron microscope evaluations. The inflammatory response of the chorioallantoic membrane to biomaterials was fully characterized and found to be similar to that of the mammalian response and was also seen to vary according to test materials. We also found that the structure and geometry of the test materials greatly influenced the incorporation of the samples in the CAM. The similarity of the tissue response of the CAM with the mammalian models, plus the low cost, simplicity, and possibility to continuously visualize the test site through the shell window make this animal model particularly attractive for the rapid in vivo screening of biomaterials.

[1]  F Moussy,et al.  In vivo evaluation of a dexamethasone/PLGA microsphere system designed to suppress the inflammatory tissue response to implantable medical devices. , 2002, Journal of biomedical materials research.

[2]  D. Klee,et al.  The chorioallantoic membrane of the chick embryo as a simple model for the study of the angiogenic and inflammatory response to biomaterials , 2001, Journal of materials science. Materials in medicine.

[3]  D. Ribatti,et al.  Angiogenesis in lipoma: An experimental study in the chick embryo chorioallantoic membrane. , 1999, International journal of molecular medicine.

[4]  P. Canfield.  Comparative cell morphology in the peripheral blood film from exotic and native animals. , 1998, Australian veterinary journal.

[5]  P. Wacnik,et al.  Open-Field and LPS-Induced Sickness Behavior in Young Chickens Effects of Embryonic Cocaine and/or Ritanserin , 1998, Pharmacology Biochemistry and Behavior.

[6]  B. Harmon,et al.  Avian heterophils in inflammation and disease resistance. , 1998, Poultry science.

[7]  N. Jain,et al.  Effects of anti-inflammatory drugs on increased vascular permeability in acute inflammatory response in the chicken. , 1995, Avian pathology : journal of the W.V.P.A.

[8]  W. Risau,et al.  Overexpression of vascular endothelial growth factor in the avian embryo induces hypervascularization and increased vascular permeability without alterations of embryonic pattern formation. , 1995, Developmental biology.

[9]  D. J. Harrison,et al.  In vitro and in vivo performance and lifetime of perfluorinated ionomer-coated glucose sensors after high-temperature curing. , 1994, Analytical chemistry.

[10]  U. Katz,et al.  Ion transport across the early chick embryo: II. Characterization and pH sensitivity of the transembryonic short-circuit current , 1994, The Journal of Membrane Biology.

[11]  K. Klasing.  Avian leukocytic cytokines. , 1994, Poultry science.

[12]  B. Christ,et al.  In vivo effects of vascular endothelial growth factor on the chicken chorioallantoic membrane , 1993, Cell and Tissue Research.

[13]  G. Birchard,et al.  A COMPARISON OF AVIAN AND REPTILIAN CHORIOALLANTOIC VASCULAR DENSITY , 1993 .

[14]  K. Klasing.  Avian inflammatory response: mediation by macrophages. , 1991, Poultry science.

[15]  K. Spanel‐Borowski,et al.  THE CHICK CHORIOALLANTOIC MEMBRANE ASSAY IN THE ASSESSMENT OF ANGIOGENIC FACTORS , 1988 .

[16]  K. Klasing.  Avian interleukin-1: immunological and physiological functions , 1987 .

[17]  G. N. Kolte,et al.  Studies on acute inflammation in the chicken using mesentery as a test system. , 1980, Research in veterinary science.

[18]  S. Dunham,et al.  Hen's eggs with retarded gas exchange. I. Chorioallantoic capillary growth. , 1979, Respiration physiology.

[19]  H. C. Carlson.  The acute inflammatory reaction in chicken breast muscle. , 1972, Avian diseases.

[20]  Jortner Bs,et al.  Turpentine-induced inflammation in the chicken. A light- and electron- microscope study, with emphasis on the macrophage, epithelioid cell, and multinucleated giant cell reaction. , 1971 .

[21]  Moore Ge.  The Spread of Cancer , 1970 .

[22]  M. Verneuil.  The Spread of Cancer , 1877, Edinburgh medical journal.

[23]  I. Flamme,et al.  The in vivo activity of vascular endothelial growth factor isoforms in the avian embryo. , 1998, Growth factors.

[24]  K. Spanel‐Borowski,et al.  The chick chorioallantoic membrane as test system for biocompatible materials , 1989, Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie.

[25]  H. Shepard,et al.  Macrophage-induced angiogenesis is mediated by tumour necrosis factor-α , 1987, Nature.

[26]  K. Jentzsch,et al.  The chick embryo choriallantoic membrane as a bioassay for angiogenesis factors: reactions induced by carrier materials. , 1978, Experimentelle Pathologie.