In-vitro susceptibility, tolerance and glycocalyx production in Streptococcus mutans.

We studied the presence of high-level resistance to aminoglycosides, penicillin tolerance and glycocalyx production in 160 isolates of Streptococcus mutans. Susceptibility to amoxycillin, cefazolin, imipenem, erythromycin, clindamycin, vancomycin and teicoplanin was also investigated. Of the isolates analysed, 58.8% produced glycocalyx in vitro and 2.5% were penicillin-tolerant. High-level resistance to streptomycin was found in 16.3% of the isolates, but all were sensitive to all other antibiotics tested. We found no significant relationship between glycocalyx production and high-level streptomycin resistance, penicillin tolerance or antibiotic susceptibility, except for a greater susceptibility to clindamycin and vancomycin in isolates that produced glycocalyx. Although our findings reflect the clinically favourable pattern of susceptibility currently found in this species, the appearance in some isolates of resistance, tolerance and glycocalyx production should be investigated because of the risks involved in endocarditis caused by S. mutans.

[1]  B. Herndon,et al.  Reactivity of the glycocalyx of endocarditis-producing viridans group streptococci. , 1993, Diagnostic microbiology and infectious disease.

[2]  J. Gutiérrez,et al.  In vitro activity of macrolides and lincosamides against oral streptococci: a therapeutic alternative in prophylaxis for infective endocarditis. , 1993, International journal of antimicrobial agents.

[3]  H. C. Wu,et al.  [Morphologic and biochemical characteristics of viridans streptococci isolated from dental plaque]. , 1992, Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology.

[4]  P. Serra,et al.  4-week treatment of streptococcal native valve endocarditis with high-dose teicoplanin , 1992, Antimicrobial Agents and Chemotherapy.

[5]  G. Piédrola,et al.  Antimicrobial susceptibility of 1042 strains of Streptococcus mutans and Streptococcus sobrinus: comparison from 1985 to 1989. , 1991, Oral microbiology and immunology.

[6]  I. van de Rijn,et al.  Analysis of adherence of Streptococcus defectivus and endocarditis-associated streptococci to extracellular matrix , 1991, Infection and Immunity.

[7]  D. G. White,et al.  Incidence of penicillin tolerance among blood culture isolates of Streptococcus sanguis, 1987-88. , 1991, Journal of clinical pathology.

[8]  B. Herndon,et al.  Association of cell-adherent glycocalyx and endocarditis production by viridans group streptococci , 1990, Journal of clinical microbiology.

[9]  B. Herndon,et al.  Clindamycin effect on glycocalyx production in experimental viridans streptococcal endocarditis. , 1990, The Journal of infectious diseases.

[10]  P. James.  Comparison of four methods for the determination of MIC and MBC of penicillin for viridans streptococci and the implications for penicillin tolerance. , 1990, The Journal of antimicrobial chemotherapy.

[11]  D. Greenwood,et al.  Differential response to benzylpenicillin in vivo of tolerant and non-tolerant variants of Streptococcus sanguis II. , 1990, The Journal of antimicrobial chemotherapy.

[12]  R. Freeman,et al.  Tolerance to penicillin in streptococci of viridans group , 1989, Journal of clinical pathology.

[13]  B. Herndon,et al.  Quantitative assay of glycocalyx produced by viridans group streptococci that cause endocarditis , 1989, Journal of clinical microbiology.

[14]  K. Dornbusch,et al.  In-vitro activity of the new penems FCE 22101 and FCE 24362 alone or in combination with aminoglycosides against streptococci isolated from patients with endocarditis. , 1989, The Journal of antimicrobial chemotherapy.

[15]  W. P. Holbrook,et al.  Penicillin tolerance among oral streptococci. , 1988, Journal of medical microbiology.

[16]  B. Cunha,et al.  Streptococcus mutans vertebral osteomyelitis. , 1988, Heart & lung : the journal of critical care.

[17]  B. Cunha,et al.  Streptococcus mutans endocarditis: report of three cases and review of the literature. , 1988, Heart & lung : the journal of critical care.

[18]  J. Etienne,et al.  Susceptibilities of streptococcal strains associated with infective endocarditis to nine antibiotics. , 1988, Chemotherapy.

[19]  J. Mills,et al.  Enzymatic modification of glycocalyx in the treatment of experimental endocarditis due to viridans streptococci. , 1987, The Journal of infectious diseases.

[20]  D. Shanson,et al.  Comparison of intravenous teicoplanin with intramuscular amoxycillin for the prophylaxis of streptococcal bacteraemia in dental patients. , 1987, The Journal of antimicrobial chemotherapy.

[21]  W. Wilson,et al.  In vitro and in vivo studies of streptomycin-resistant, penicillin-susceptible streptococci from patients with infective endocarditis. , 1987, The Journal of infectious diseases.

[22]  J. Williams,et al.  Transferable resistance and aminoglycoside-modifying enzymes in enterococci. , 1985, Journal of medical microbiology.

[23]  R. Greenman,et al.  Efficacy of imipenem/cilastatin in endocarditis. , 1985, The American journal of medicine.

[24]  M. Sande,et al.  Penicillin therapy for treatment of experimental endocarditis caused by viridans streptococci in animals. , 1985, The Journal of infectious diseases.

[25]  G. K. Ingalls.  Detection of penicillin tolerance in streptococci , 1985 .

[26]  L. Pulliam,et al.  Effects of exopolysaccharide production by viridans streptococci on penicillin therapy of experimental endocarditis. , 1985, The Journal of infectious diseases.

[27]  D. Shanson,et al.  Erythromycin stearate, 1.5 g, for the oral prophylaxis of streptococcal bacteraemia in patients undergoing dental extraction: efficacy and tolerance. , 1985, The Journal of antimicrobial chemotherapy.

[28]  D. Horstkotte,et al.  [Therapy and prevention of infectious endocarditis]. , 1984, Schweizerische medizinische Wochenschrift.

[29]  J. Etienne,et al.  Antibiotic susceptibility of streptococcal strains associated with infective endocarditis. , 1984, European heart journal.

[30]  C. Carlier,et al.  Characterization of high-level aminoglycoside resistance in a strain of Streptococcus pneumoniae. , 1984, Journal of general microbiology.

[31]  S. Aragon.  Successful prophylaxis against experimental streptococcal endocarditis with bacteriostatic antibiotics , 1984 .

[32]  A. W. Anderson,et al.  Endocarditis due to viridans-type streptococci tolerant to beta-lactam antibiotics: therapeutic problems. , 1982, British medical journal.

[33]  B. Yersin,et al.  Effect of nitrogen mustard on natural history of right-sided streptococcal endocarditis in rabbits: role for cellular host defenses , 1982, Infection and immunity.

[34]  J. Dankert,et al.  PENICILLIN TOLERANCE AND BACTERIAL ENDOCARDITIS , 1980, The Lancet.

[35]  E. Bruck,et al.  National Committee for Clinical Laboratory Standards. , 1980, Pediatrics.

[36]  W. Wilson,et al.  Antimicrobial susceptibilities of species of viridans streptococci. , 1979, The Journal of infectious diseases.

[37]  C. Watanakunakorn,et al.  Synergism with aminoglycosides of penicillin, ampicillin and vancomycin against non-enterococcal group-D streptococci and viridans streptococci. , 1977, Journal of medical microbiology.

[38]  J. Ferretti,et al.  Susceptibility of Streptococcus mutans to Antimicrobial Agents , 1976, Antimicrobial Agents and Chemotherapy.

[39]  M. Sande,et al.  Role of the Vegetation in Experimental Streptococcus viridans Endocarditis , 1974, Infection and immunity.

[40]  C. Baker,et al.  Antimicrobial Susceptibility of Streptococcus mutans Isolated from Patients with Endocarditis , 1974, Antimicrobial Agents and Chemotherapy.