Expression of c‐fos protein in interneurons and projection neurons of the rat spinal cord in response to noxious somatic, articular, and visceral stimulation

This study used immunocytochemistry to examine the pattern of noxious‐stimulus evoked expression of the proto‐oncogene c‐fos in the spinal cord of the rat. Both noxious somatic and joint stimulation in awake rats evoked the expression of c‐fos protein in similar areas of the lumbar spinal cord. C‐fos‐immunoreactive neurons were found in laminae I and outer II, in the lateral part of the neck of the dorsal horn, and in laminae VII, VIII, and X. All of the labelled neurons were located ipsilateral to the injured hindpaw, except for lamina VIII where bilateral labelling was recorded. The c‐fos‐immunoreactive neurons in lamina I extended from the L3 segment to the rostral sacral cord; staining in outer lamina II was only found at the L4 segment. The more deeply located cells, of the dorsal and medioventral horns, had the most extensive rostrocaudal spread; they were found from L1 through the rostral sacral segments.

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