The binding of zinc to glycerol-extracted muscle, and its relaxing effect.

. The binding of zinc to glycerol-extracted rabbit psoas muscle has been correlated with the zinc + ATP-induced relaxation. The minimum amount of zinc that must be absorbed by the glycerol-extracted fibres for complete isometric relaxation is about 3–4 mmoles per kg dry weight, which is close to the amount lost during glycerol-extraction. The extracted fibres are able to absorb zinc in great excess of the concentration necessary for complete relaxation. The pH-dependence of the contraction-inhibiting effect of zinc and of the binding of zinc in high concentrations to the muscle shows that groups with a pK-value of about 7, probably imidazole groups, are involved in both cases. The finding of an additive synergism between the effects of zinc and mersalyl suggests that also SH-groups may be involved in the functionally relevant coupling of zinc to the contractile element.

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