论文信息 - Selection of spermatozoa with normal nuclei to improve the pregnancy rate with intracytoplasmic sperm injection.

Selection of spermatozoa with normal nuclei to improve the pregnancy rate with intracytoplasmic sperm injection.

To the Editor: Intracytoplasmic injection of sperm is the recommended treatment for male infertility, associated with an average pregnancy rate per cycle of about 30 percent.1 Although sperm count and motility were found to have no effect on the outcome of intracytoplasmic sperm injection,2 scanning and transmission electron microscopy indicated that the achievement of pregnancy may depend on normal morphology of the sperm nucleus.3 We therefore introduced the motile-sperm organelle-morphology examination (MSOME), by which the fine nuclear morphology of motile spermatozoa is examined in real time with an inverted light microscope equipped with high-power differential interference contrast (Nomarski/DIC) optics (magnification, . . .

[1] Y. Beyth,et al. ART success and in vivo sperm cell selection depend on the ultramorphological status of spermatozoa , 1999, Andrologia.

[2] K. Nygren,et al. Assisted reproductive technology in Europe, 1997. Results generated from European registers by ESHRE. European IVF-Monitoring Programme (EIM), for the European Society of Human Reproduction and Embryology (ESHRE). , 2001, Human reproduction.

[3] Z. Nagy,et al. Special applications of intracytoplasmic sperm injection: the influence of sperm count, motility, morphology, source and sperm antibody on the outcome of ICSI. , 1998, Human reproduction.

[4] A. Wetzels,et al. Cumulative probability of achieving an ongoing pregnancy after in-vitro fertilization and intracytoplasmic sperm injection according to a woman's age, subfertility diagnosis and primary or secondary subfertility. , 2000, Human reproduction.