Comparison of Bone Marrow Cells Harvested from Various Bones of Cynomolgus Monkeys at Various Ages by Perfusion or Aspiration Methods: A Preclinical Study for Human BMT

Using cynomolgus monkeys, we have previously established a new method for harvesting bone marrow cells (BMCs) with minimal contamination of the BMCs with T cells from the peripheral blood. We originally conducted this new “perfusion method” in the long bones (the humerus, femur, and tibia) of cynomolgus monkeys.

[1]  J. Hansen,et al.  Analysis of 462 transplantations from unrelated donors facilitated by the National Marrow Donor Program. , 1993, The New England journal of medicine.

[2]  C. Ricordi,et al.  Analysis of post-transplant immune status in recipients of liver/bone marrow allografts. , 1999, Human immunology.

[3]  R. Colvin,et al.  Modifications of the conditioning regimen for achieving mixed chimerism and donor-specific tolerance in cynomolgus monkeys. , 1997, Transplantation.

[4]  A. Marmont,et al.  Immune ablation followed by allogeneic or autologous bone marrow transplantation: A new treatment for severe autoimmune diseases? , 1994, Stem cells.

[5]  S. Ikehara,et al.  Intra-bone marrow injection of allogeneic bone marrow cells: a powerful new strategy for treatment of intractable autoimmune diseases in MRL/lpr mice. , 2001, Blood.

[6]  S. Ikehara,et al.  Treatment of autoimmune diseases by hematopoietic stem cell transplantation. , 2001, Experimental hematology.

[7]  K. Sullivan,et al.  Chronic graft-versus-host disease in 52 patients: adverse natural course and successful treatment with combination immunosuppression. , 1981, Blood.

[8]  Simon C Watkins,et al.  Phenotypic characterization of a novel bone marrow-derived cell that facilitates engraftment of allogeneic bone marrow stem cells. , 1994, Blood.

[9]  D. Sachs,et al.  Mixed chimerism as an approach to transplantation tolerance. , 2000, Clinical immunology.

[10]  Thomas Ed,et al.  DOES BONE MARROW TRANSPLANTATION CONFER A NORMAL LIFE SPAN , 1999 .

[11]  S. Ikehara,et al.  Major histocompatibility complex restriction between hematopoietic stem cells and stromal cells in vivo. , 1997, Blood.

[12]  K. Verbanac,et al.  The Facilitating Effect Of One‐Dr Antigen Sharing In Renal Allograft Tolerance Induced By Donor Bone Marrow In Rhesus Monkeys , 1995, Transplantation.

[13]  S. Ikehara,et al.  A New Method for Bone Marrow Cell Harvesting , 2000, Stem cells.

[14]  E D Thomas,et al.  Does bone marrow transplantation confer a normal life span? , 1999, The New England journal of medicine.

[15]  I. Weissman,et al.  CD8+TCR+ and CD8+TCR- cells in whole bone marrow facilitate the engraftment of hematopoietic stem cells across allogeneic barriers. , 1999, Immunity.

[16]  S. Ikehara,et al.  Treatment of intractable autoimmune diseases in MRL/lpr mice using a new strategy for allogeneic bone marrow transplantation. , 2000, Blood.

[17]  S. Ikehara,et al.  Requirement of donor-derived stromal cells in the bone marrow for successful allogeneic bone marrow transplantation. Complete prevention of recurrence of autoimmune diseases in MRL/MP-Ipr/Ipr mice by transplantation of bone marrow plus bones (stromal cells) from the same donor. , 1994, Journal of immunology.

[18]  M. Kawamura,et al.  Successful bone marrow transplantation by bone grafts in chimeric-resistant combination. , 1995, Experimental hematology.

[19]  Pepe,et al.  Marrow transplantation from unrelated donors for treatment of hematologic malignancies: effect of mismatching for one HLA locus. , 1993, Blood.

[20]  Graft-versus-host disease. , 1991 .

[21]  R Storb,et al.  Technique for human marrow grafting. , 1970, Blood.

[22]  K. Matsuda,et al.  Development of a modified selective amplifier gene for hematopoietic stem cell gene therapy , 1999, Gene Therapy.

[23]  S. Ikehara,et al.  Successful Allogeneic Bone Marrow Transplantation (BMT) by Injection of Bone Marrow Cells via Portal Vein: Stromal Cells as BMT‐Facilitating Cells , 2001, Stem cells.