论文信息 - Isolation of symbiotic bacteria and bioactive proteins from the marine sponge, Callyspongia diffusa

Isolation of symbiotic bacteria and bioactive proteins from the marine sponge, Callyspongia diffusa

. Both extracts exhibited hemolytic activity on chicken erythrocytes at 6.99 HT/mg for methanolic and 8.64 HT/mg for aqueous extracts. On SDS-PAGE, the crude protein yielded eight bands in methanolic extract and five bands in aqueous extract, with molecular weight ranging from 14.4 to 116 kDa with three well defined bands of 19.5, 39.0, 66.2 kDa in both the extracts.

K. Kathiresan | T. T. A. Kumar | S. Boobathy

[1] K. Kathiresan,et al. Bioprospecting of marine organisms for novel bioactive compounds , 2008 .

[2] K. Steube,et al. Swinhoeiamide A, a new highly active calyculin derivative from the marine sponge Theonella swinhoei. , 2002, Journal of natural products.

[3] Yamamoto,et al. Roles of dopamine and serotonin in larval attachment of the barnacle, Balanus amphitrite. , 1999, The Journal of experimental zoology.

[4] A. Leonard,et al. Metabolites from an Antarctic sponge-associated bacterium, Pseudomonas aeruginosa. , 1996, Journal of natural products.

[5] N. Fusetani. Bioactive substances from marine sponges , 1996 .

[6] P. R. Bergquist,et al. The incidence of antibacterial activity in marine demospongiae; systematic and geographic considerations , 1978 .

[7] R. E. Buchanan,et al. Bergey's Manual of Determinative Bacteriology. , 1975 .

[8] G. J. Bakus,et al. Toxicity in Sponges and Holothurians: A Geographic Pattern , 1974, Science.

[9] P. Burkholder. 5. – THE ECOLOGY OF MARINE ANTIBIOTICS AND CORAL REEFS , 1973 .

[10] U. K. Laemmli,et al. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[11] S. T. Cowan. Bergey's Manual of Determinative Bacteriology , 1948, Nature.

[12] O. H. Lowry,et al. The determination of inorganic phosphate in the presence of labile phosphate esters. , 1946, The Journal of biological chemistry.