In vivo Studies oft the Synthesis of Alginic Acid in Ishige okamurai

Ishige okamurai was subjected to the chase experiments in rdation to the biosynthesis of alginatcs. During C-photo assimiiation, rates of thc C-incorpotation into three kinds of alginate preparatiuns were in the ordcr of M-rich MG> G-rich ones. In the chase cuiture, the radioactivities of these alginatcs changed in cach charactcristic pattern At earher stages of the chase cuiture, thc radioactivities of these alginates increased, both in the light and dark and the increasing rates wete the same order äs the above, but those of M-rich alginate began to decrtasc most rapidlv while those of G-rich alginate continued to increase until later stages of thc cuiture tcstcd. Für thc first 24-hour cliasc cuiture, radioactivity of M-residues was far larger in the light than in the dark with thc three alginatc prcparations but the Situation was reversed during the subsequent 24-hours and thereafter. In contrast, radioact ivi ty of G-rcsiducs v äs aiways larget in the dark than in the light with the three alginate preparations throughout thc chase pcriods.