Federal Integrated Biotreatment Research Consortium (FIBRC): Flask to Field Initiative

Abstract : The Federal Integrated Biotreatment Research Consortium (Flask to Field) represented a 7-year Strategic Environmental Research and Development Program (SERDP)-funded effort by several research laboratories to develop bioremediation technologies for contaminated U.S. Department of Defense (DoD) sites. The project was proposed as a new 6.2-level research program to enhance, but not duplicate, the existing funded efforts in the DoD. It also complemented both the U.S. Environmental Protection Agency and U.S. Department of Energy short- and long-term research strategies. The primary objective of this project was to develop the most promising biotreatment processes at the bench, intermediate, and pilot scale in each of four thrust areas: polycyclic aromatic hydrocarbons (PAHs), chlorinated solvents, energetics and explosives, and polychlorinated biphenyls (PCBs). The consortium structure consisted of a lead principal investigator or director and four thrust area coordinators. Lead researchers at various government installations and academic institutions carried out the individual projects in each thrust area. Engineering groups worked closely with scientists in evaluating the potential of the resulting technologies and in the transfer of technologies from bench scale to field. A technical advisory committee consisting of leading biotechnology specialists in academia, industry, consulting, and government assisted and advised the director and reviewed individual projects for technical merit.

[1]  R. Crawford,et al.  Novel biotransformations of 4-chlorobiphenyl by a Pseudomonas sp , 1988, Applied and environmental microbiology.

[2]  E. Stackebrandt,et al.  Partial and complete 16S rDNA sequences, their use in generation of 16S rDNA phylogenetic trees and their implications in molecular ecological studies , 1995 .

[3]  D. Abramowicz.  Aerobic and Anaerobic Biodegradation of PCBs: A Review , 1990 .

[4]  Genetic control of degradation of chlorinated benzoic acids in Arthrobacter globiformis, Corynebacterium sepedonicum and Pseudomonas cepacia strains. , 1991, FEMS microbiology letters.

[5]  M. Nishiyama,et al.  Development of a host-vector system in a Rhodococcus strain and its use for expression of the cloned nitrile hydratase gene cluster. , 1992, Journal of general microbiology.

[6]  Russell Higuchi,et al.  Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions , 1993, Bio/Technology.

[7]  D. Yeh,et al.  Toxicity and biodegradability screening of nonionic surfactants using sediment-derived methanogenic consortia , 1998 .

[8]  G. W. Sewell,et al.  Anaerobic bioremediation of chlorinated ethenes , 1999 .

[9]  W. Williams.  Microbial reductive dechlorination of polychlorinated biphenyls in heat treated and bromoethanesulfonate treated anaerobic sediment slurries , 1994 .

[10]  S. P. Neuman.  Analysis of Pumping Test Data From Anisotropic Unconfined Aquifers Considering Delayed Gravity Response , 1975 .

[11]  G S Sayler,et al.  Monitoring of naphthalene catabolism by bioluminescence with nah-lux transcriptional fusions , 1990, Journal of bacteriology.

[12]  L. Abriola,et al.  Solubilization of Dodecane, Tetrachloroethylene, and 1,2-Dichlorobenzene in Micellar Solutions of Ethoxylated Nonionic Surfactants , 1997 .

[13]  K. Furukawa,et al.  Effect of chlorine substitution on the bacterial metabolism of various polychlorinated biphenyls , 1979, Applied and environmental microbiology.

[14]  K. Furukawa,et al.  Metabolism of 2,4,4'-trichlorobiphenyl by Acinetobacter sp. P6. , 1979 .

[15]  M. Fukuda,et al.  A Novel Transformation of Polychlorinated Biphenyls by Rhodococcus sp. Strain RHA1 , 1995, Applied and environmental microbiology.

[16]  S. Safe.  Toxicology, structure-function relationship, and human and environmental health impacts of polychlorinated biphenyls: progress and problems. , 1993, Environmental health perspectives.

[17]  P. Mccarty,et al.  Biologically enhanced dissolution of tetrachloroethene DNAPL , 2000 .

[18]  Sujoy B. Roy,et al.  Solvent extraction for remediation of coal tar sites. Final report , 1992 .

[19]  L. Dölken,et al.  Quantitative detection of t(14;18)-positive cells by real-time quantitative PCR using fluorogenic probes. , 1998, BioTechniques.

[20]  J. Ezzell,et al.  5′ Nuclease PCR Assay To DetectYersinia pestis , 1998, Journal of Clinical Microbiology.

[21]  Jeff J. Morse,et al.  A Treatability Test for Evaluating the Potential Applicability of the Reductive Anaerobic Biological In Situ Treatment Technology (RABITT) to Remediate Chloroethenes , 1998 .

[22]  J. Gossett,et al.  Reductive dehalogenation of chlorinated ethenes and halogenated ethanes by a high-rate anaerobic enrichment culture. , 1994, Environmental science & technology.

[23]  S. C. Young,et al.  Application of the electromagnetic borehole flowmeter , 1998 .

[24]  D. Yeh,et al.  Effect of Tween surfactants on methanogenesis and microbial reductive dechlorination of hexachlorobenzene , 1999 .

[25]  A. L. Wood,et al.  Remediation of soils and aquifers contaminated with hydrophobic organic chemicals: theoretical basis for the use of cosolvents , 1997 .

[26]  C. A. Fewson,et al.  Biotransformations catalyzed by the genus Rhodococcus. , 1994, Critical reviews in biotechnology.

[27]  J. F. Brown,et al.  Extensive degradation of Aroclors and environmentally transformed polychlorinated biphenyls by Alcaligenes eutrophus H850 , 1987, Applied and environmental microbiology.

[28]  K. Timmis,et al.  Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Pseudomonas sp. strain LB400 , 1995, Applied and environmental microbiology.

[29]  B. Kueper,et al.  Manipulation of density and viscosity for the optimization of DNAPL recovery by alcohol flooding , 1999 .

[30]  M. Fukuda,et al.  Characterization of biphenyl catabolic genes of gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1 , 1995, Applied and environmental microbiology.

[31]  M. Fukuda,et al.  The bphDEF meta-cleavage pathway genes involved in biphenyl/polychlorinated biphenyl degradation are located on a linear plasmid and separated from the initial bphACB genes in Rhodococcus sp. strain RHA1. , 1997, Gene.

[32]  J. Suflita,et al.  Anaerobic biotransformation of contaminants in the subsurface. Environmental research brief , 1991 .

[33]  J. Quensen,et al.  Development of a Rhodococcus recombinant strain for degradation of products from anaerobic dechlorination of PCBs. , 2001, Environmental science & technology.

[34]  E. G. Plotnikova,et al.  Cloning, Expression, and Nucleotide Sequence of thePseudomonas aeruginosa 142 ohb Genes Coding for Oxygenolytic ortho Dehalogenation of Halobenzoates , 1999, Applied and Environmental Microbiology.

[35]  Kenneth R. Morris,et al.  Solubility of aromatic pollutants in mixed solvents , 1988 .

[36]  E. G. Plotnikova,et al.  Cloning and expression of the Arthrobacter globiformis KZT1 fcbA gene encoding dehalogenase (4-chlorobenzoate-4-hydroxylase) in Escherichia coli. , 1991, FEMS microbiology letters.

[37]  A. L. Wood,et al.  Evaluation of in situ cosolvent flushing dynamics using a network of spatially distributed multilevel samplers , 1998 .

[38]  R. Hausinger,et al.  NADPH-dependent reductive ortho dehalogenation of 2,4-dichlorobenzoic acid in Corynebacterium sepedonicum KZ-4 and Coryneform bacterium strainNTB-1 via 2,4-dichlorobenzoyl coenzyme A , 1996, Journal of bacteriology.

[39]  D. Helinski,et al.  Regions of broad-host-range plasmid RK2 involved in replication and stable maintenance in nine species of gram-negative bacteria , 1985, Journal of bacteriology.

[40]  Pat Phibbs.  News: EPA reassessment finds as much as 20 times less cancer risk from PCBs , 1996 .

[41]  Thermal Blanket for In-Situ Remediation of Surficial Contamination: A Pilot Test , 1996 .

[42]  J. Duinker,et al.  Complete characterization of polychlorinated biphenyl congeners in commercial Aroclor and Clophen mixtures by multidimensional gas chromatography-electron capture detection , 1989 .

[43]  A. L. Wood,et al.  Field‐scale evaluation of in situ cosolvent flushing for enhanced aquifer remediation , 1997 .

[44]  T. Omori,et al.  Purification and Some Properties of 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid(HOPDA) Reducing Enzyme from Pseudomonas cruciviae S93B1 Involved in the Degradation of Biphenyl , 1986 .

[45]  G. Kowalchuk,et al.  Denaturing gradient gel electrophoresis (DGGE) in microbial ecology. , 2004 .

[46]  D. Focht,et al.  Construction of a Novel Polychlorinated Biphenyl-Degrading Bacterium: Utilization of 3,4′-Dichlorobiphenyl by Pseudomonas acidovorans M3GY , 1994, Applied and environmental microbiology.

[47]  W. Reineke,et al.  Suicide Inactivation of Catechol 2,3-Dioxygenase from Pseudomonas putida mt-2 by 3-Halocatechols , 1984, Applied and environmental microbiology.

[48]  D. Focht,et al.  Mineralization of 2-chloro- and 2,5-dichlorobiphenyl by Pseudomonas sp. strain UCR2. , 1992, FEMS microbiology letters.

[49]  C. Peters,et al.  Coal tar dissolution in water-miscible solvents : experimental evaluation , 1993 .

[50]  G. W. Sewell,et al.  Stimulation of Reductive Dechlorination of Tetrachloroethene in Anaerobic Aquifer Microcosms by Addition of Short-Chain Organic Acids or Alcohols , 1992, Applied and environmental microbiology.

[51]  R. Luthy,et al.  Aromatic Compound Solubility in Solvent/Water Mixtures , 1986 .

[52]  Y. Zo,et al.  Nonradioactive method to study genetic profiles of natural bacterial communities by PCR-single-strand-conformation polymorphism , 1996, Applied and environmental microbiology.

[53]  M. D. Perkins,et al.  Comparison of the ABI 7700 System (TaqMan) and Competitive PCR for Quantification of IS6110 DNA in Sputum during Treatment of Tuberculosis , 1998, Journal of Clinical Microbiology.

[54]  E. Delong,et al.  Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells. , 1989, Science.

[55]  K. Furukawa,et al.  Effect of Chlorine Substitution on the Biodegradability of Polychlorinated Biphenyls , 1978, Applied and environmental microbiology.

[56]  V Lang,et al.  Polychlorinated biphenyls in the environment. , 1992, Journal of chromatography.

[57]  K. Timmis,et al.  Genetic analysis of a Pseudomonas locus encoding a pathway for biphenyl/polychlorinated biphenyl degradation. , 1993, Gene.

[58]  J. Gossett,et al.  Reductive dechlorination of high concentrations of tetrachloroethene to ethene by an anaerobic enrichment culture in the absence of methanogenesis , 1991, Applied and environmental microbiology.

[59]  D. Brandes,et al.  Importance of phase behavior on the removal of residual DNAPLs from porous media by alcohol flooding , 1993 .

[60]  D. Focht,et al.  Degradation of polychlorinated biphenyls by two species of Achromobacter. , 1973, Canadian journal of microbiology.

[61]  M. Fukuda,et al.  Growth inhibition of Rhodococcus sp. strain RHA1 in the course of PCB transformation , 1996, Biotechnology Letters.

[62]  D. Bedard,et al.  Evidence for novel mechanisms of polychlorinated biphenyl metabolism in Alcaligenes eutrophus H850 , 1987, Applied and environmental microbiology.

[63]  J. Quensen,et al.  Degradation of anaerobic reductive dechlorinationproducts of Aroclor 1242 by four aerobic bacteria , 2004, Biodegradation.

[64]  A. Abdul,et al.  In Situ Surfactant Washing of Polychlorinated Biphenyls and Oils from a Contaminated Field Site: Phase II Pilot Study , 1994 .

[65]  R. Kolter,et al.  Green fluorescent protein as a marker for Pseudomonas spp , 1997, Applied and environmental microbiology.

[66]  D. Focht,et al.  Genetic construction of PCB degraders , 1994, Biodegradation.

[67]  F. Messier,et al.  A comprehensive gas chromatographic/mass spectrometric analysis of 4‐chlorobiphenyl bacterial degradation products , 1989 .

[68]  D. Ghosal,et al.  Operon structure and nucleotide homology of the chlorocatechol oxidation genes of plasmids pJP4 and pAC27. , 1989, Gene.

[69]  P. Suresh C. Rao,et al.  Remediation of Contaminated Soils by Solvent Flushing , 1994 .

[70]  P. Rao,et al.  Evaluation of In-Situ DNAPL Remediation and Innovative Site Characterization Techniques , 2000 .

[71]  M. Zappi,et al.  Evaluation of Bioslurry Ecosystems for Removal of TNT from Contaminated Soil Using a Variety of Process Amendments a , 1997 .

[72]  O. Hutzinger,et al.  Chemistry of PCB's , 1974 .

[73]  C. T. Miller,et al.  Cosolvent-enhanced remediation of residual dense nonaqueous phase liquids: experimental investigation. , 1995, Environmental science & technology.

[74]  K. Livak,et al.  Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes , 1995, Applied and environmental microbiology.

[75]  R. Hausinger,et al.  Pseudomonas aeruginosa 142 uses a three-component ortho-halobenzoate 1,2-dioxygenase for metabolism of 2,4-dichloro- and 2-chlorobenzoate , 1994, Journal of bacteriology.

[76]  B. Fathepure,et al.  Reductive dechlorination of perchloroethylene and the role of methanogens , 1988 .

[77]  J. Quensen,et al.  Construction and Characterization of Two Recombinant Bacteria That Grow on ortho- and para-Substituted Chlorobiphenyls , 1999, Applied and Environmental Microbiology.

[78]  Sujoy B. Roy,et al.  Assessment of in situ solvent extraction for remediation of coal tar sites: Column studies , 1995 .

[79]  B. Kueper,et al.  Removal of pooled dense, nonaqueous phase liquid from saturated porous media using upward gradient alcohol floods , 1997 .

[80]  J. Horton,et al.  Dihydroxylation and dechlorination of chlorinated biphenyls by purified biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400 , 1995, Journal of bacteriology.

[81]  G. W. Sewell,et al.  Effects of three concentrations of mixed fatty acids on dechlorination of tetrachloroethene in aquifer microcosms , 1994 .

[82]  D. Bedard,et al.  Rapid assay for screening and characterizing microorganisms for the ability to degrade polychlorinated biphenyls , 1986, Applied and environmental microbiology.

[83]  Jizhong Zhou,et al.  Pseudomonas stutzeri Nitrite Reductase Gene Abundance in Environmental Samples Measured by Real-Time PCR , 2001, Applied and Environmental Microbiology.

[84]  James M. Tiedje,et al.  16S rRNA Gene-Based Detection of Tetrachloroethene-Dechlorinating Desulfuromonas andDehalococcoides Species , 2000, Applied and Environmental Microbiology.

[85]  J. Haddock,et al.  Influence of Chlorine Substituents on Rates of Oxidation of Chlorinated Biphenyls by the Biphenyl Dioxygenase ofBurkholderia sp. Strain LB400 , 2000, Applied and Environmental Microbiology.

[86]  S. J. Flynn,et al.  Microbial Community Changes Associated with a Shift from Reductive Dechlorination of PCE to Reductive Dechlorination of cis-DCE and VC , 2000 .

[87]  R. Falta,et al.  Swelling of DNAPL by Cosolvent Flooding to allow its Removal as an LNAPL , 1996 .

[88]  Paul D. Jones,et al.  A comparison of water quality criteria for the Great Lakes based on human and wildlife health , 1993 .

[89]  Calvin R. Brunner.  Handbook of Incineration Systems , 1991 .

[90]  M. Röllinghoff,et al.  Quantitative Detection of Borrelia burgdorferi by Real-Time PCR , 1999, Journal of Clinical Microbiology.

[91]  R. Lenski,et al.  Genetic analysis of a plasmid-encoded, host genotype-specific enhancement of bacterial fitness , 1994, Journal of bacteriology.

[92]  J. Jawitz,et al.  In-situ alcohol flushing of a DNAPL source zone at a dry cleaner site. , 2000 .

[93]  K. Livak,et al.  Real time quantitative PCR. , 1996, Genome research.

[94]  W. Reineke,et al.  Total degradation of various chlorobiphenyls by cocultures and in vivo constructed hybrid pseudomonads. , 1991, FEMS microbiology letters.

[95]  John A. Cherry,et al.  Groundwater contamination: pump-and-treat remediation , 1989 .

[96]  G. Shreve,et al.  Reductive dechlorination of polychlorinated biphenyls in landfill leachate , 2003 .

[97]  J. Tiedje,et al.  Characterization of the Dominant and Rare Members of a Young Hawaiian Soil Bacterial Community with Small-Subunit Ribosomal DNA Amplified from DNA Fractionated on the Basis of Its Guanine and Cytosine Composition , 1998, Applied and Environmental Microbiology.

[98]  A. Chianese,et al.  Removal of PCBS from mineral oils , 1997 .

[99]  J. Quensen,et al.  Evaluation of strains isolated by growth on naphthalene and biphenyl for hybridization of genes to dioxygenase probes and polychlorinated biphenyl-degrading ability , 1996, Applied and environmental microbiology.

[100]  T. Gibson,et al.  In Situ Surfactant Washing of Polychlorinated Biphenyls and Oils from a Contaminated Site , 1992 .

[101]  J. Quensen,et al.  2-Bromoethanesulfonate, Sulfate, Molybdate, and Ethanesulfonate Inhibit Anaerobic Dechlorination of Polychlorobiphenyls by Pasteurized Microorganisms , 1999, Applied and Environmental Microbiology.

[102]  R. Lenski.  Quantifying fitness and gene stability in microorganisms. , 1991, Biotechnology.

[103]  A. L. Wood,et al.  Field evaluation of the solvent extraction residual biotreatment technology. , 2003, Environmental Science and Technology.

[104]  A. Uitterlinden,et al.  Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA , 1993, Applied and environmental microbiology.