4Hz mechanical vibration relieves pain through Na+/K+-ATPase α3 isoform-dependent brain tissue dehydration

In this work the effect of 4Hz 30dB horizontal mechanical vibration (MV) on thermal pain threshold, hydration and [ 3 H]-ouabain binding in brain and heart muscle tissues of rats was studied. It was revealed that 4Hz MV treatment for 10 minutes increased pain threshold, which was accompanied by brain and heart muscle tissue dehydration. In vitro state, hydration of brain and heart muscle tissues of sham animals was increased, while in 4Hz MV-treated animals the increase of brain tissue hydration was more pronounced and heart muscle tissues were dehydrated. The fact that 4Hz MV treatment also impacted heart muscle tissue hydration indicates that 4Hz MV effect on brain and heart muscle tissues is realized through a common messenger circulating in blood. The incubation of brain and heart muscle tissues in PS containing 10 -4 M and 10 -9 M ouabain led to tissue hydration in sham and 4Hz MV-treated animals. However, tissues of 4Hz MV-treated animals were less hydrated, and this hydration was accompanied by the decrease and increase of membrane receptors’ affinity at 10 -4 M and 10 -9 M ouabain concentrations, respectively. Based on the obtained data, it is suggested that pain-relieving effect of 4Hz MV is due to α 3 isoform-dependent brain tissue dehydration.

[1]  D. Sauter,et al.  Role of volume-regulated and calcium-activated anion channels in cell volume homeostasis, cancer and drug resistance , 2015, Channels.

[2]  S. Ayrapetyan,et al.  Age-Dependent Magnetic Sensitivity of Brain and Heart Tissues , 2015 .

[3]  S. Ayrapetyan,et al.  Age-dependent effect of static magnetic field on brain tissue hydration , 2014, Electromagnetic biology and medicine.

[4]  Baghdasaryan Naira,et al.  The impact of background radiation, illumination and temperature on EMF-induced changes of aqua medium properties , 2013, Electromagnetic Biology and Medicine.

[5]  S. Ayrapetyan,et al.  Bidirectional frequency-dependent effect of extremely low-frequency electromagnetic field on E. coli K-12 , 2013, Electromagnetic biology and medicine.

[6]  S. Ayrapetyan,et al.  The metabolic pathway of 4 Hz mechanical vibration-induced effect on snail heart muscle contractility , 2012, The Environmentalist.

[7]  S. Ayrapetyan,et al.  KETAMINE- INDUCED CELL DEHYDRATION AS A MECHANISM OF IT'S ANALGESIC AND ANESTHETIC EFFECTS , 2011 .

[8]  S. Ayrapetyan,et al.  THE BRAIN TISSUE DEHYDRATION AS A MECHANISM OF ANALGESIC EFFECT OF HYPERTONIC PHYSIOLOGICAL SOLUTION IN RATS , 2010 .

[9]  M. Alves-Pereira.  Infrasound and Low Frequency Noise : Quantification in Several Rural and Urban Environments , 2010 .

[10]  F. Lang.  Mechanisms and Significance of Cell Volume Regulation , 2006, Journal of the American College of Nutrition.

[11]  D. Carpenter,et al.  Low concentrations of ouabain stimulate Na/Ca exchange in neurons , 1996, Cellular and Molecular Neurobiology.

[12]  V. Arakelyan,et al.  The effect of osmotic gradients on the outward potassium current in dialyzed neurons ofHelix pomatia , 1993, Cellular and Molecular Neurobiology.

[13]  S. Ayrapetyan,et al.  Further study of the correlation between na-pump activity and membrane chemosensitivity , 1985, Cellular and Molecular Neurobiology.

[14]  S. Ayrapetyan,et al.  Autoregulation of the electrogenic sodium pump , 1984, Cellular and Molecular Neurobiology.

[15]  M. Pope,et al.  The effects of whole body vibration on humans: dangerous or advantageous? , 2003, Acta physiologica Hungarica.

[16]  Zijian Xie,et al.  Na(+)/K(+)-ATPase as a signal transducer. , 2002, European journal of biochemistry.

[17]  V A Parsegian,et al.  Osmotic stress, crowding, preferential hydration, and binding: A comparison of perspectives. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[18]  Aĭrapetian Sn,et al.  Mechanism of anesthetic action of magnetic fields , 1998 .

[19]  J. Skou.  The influence of some cations on an adenosine triphosphatase from peripheral nerves. , 1998, Journal of the American Society of Nephrology : JASN.

[20]  A. Herchuelz.  [Na/Ca exchange]. , 1996, Bulletin et memoires de l'Academie royale de medecine de Belgique.

[21]  K. Krnjević.  Cellular and synaptic actions of general anaesthetics. , 1992, General pharmacology.

[22]  G. Rychkov,et al.  Effects of water flow on transmembrane ionic currents in neurons of Helix pomatia and in squid giant axons. , 1988, Comparative biochemistry and physiology. A, Comparative physiology.

[23]  K. Koketsu,et al.  On the membrane potential independent mechanism of the sodium pump-induced inhibition of spontaneous electrical activity of japanese land snail neurons , 1984 .

[24]  S. Ayrapetyan.  ON THE PHYSIOLOGICAL SIGNIFICANCE OF THE PUMP-INDUCED CELL VOLUME CHANGES , 1981 .

[25]  S. Ayrapetyan,et al.  On the pump-induced cell volume changes , 1979 .

[26]  M. M. Varma,et al.  Biological Effects of Non-Ionizing Radiation. , 1975 .

[27]  A. Hodgkin,et al.  The influence of calcium on sodium efflux in squid axons , 1969, The Journal of physiology.

[28]  M. H. Aprison,et al.  ACETYLCHOLINE CONTENT OF DISCRETE AREAS OF THE BRAIN OBTAINED BY A NEAR‐FREEZING METHOD * , 1964, Journal of neurochemistry.