Analysis of nonpolar insertion mutations in the trfA gene of IncP plasmid RK2 which affect its broad-host-range property.

[1]  D. Helinski,et al.  The sequence encoding the 43-kilodalton trfA protein is required for efficient replication or maintenance of minimal RK2 replicons in Pseudomonas aeruginosa. , 1987, Plasmid.

[2]  Christopher M Thomas,et al.  Replication and maintenance of promiscuous plasmids of gram-negative bacteria. , 1987, Microbiological sciences.

[3]  Christopher M Thomas,et al.  Analysis of transcription from the trfA promoter of broad host range plasmid RK2 in Escherichia coli, Pseudomonas putida, and Pseudomonas aeruginosa. , 1987, Plasmid.

[4]  D. Figurski,et al.  Genetic basis of a Tn7 insertion mutation in the trfA region of the promiscuous IncP-1 plasmid R18 which affects its host range. , 1987, Plasmid.

[5]  Christopher M Thomas.  Evidence for the involvement of the incC locus of broad host range plasmid RK2 in plasmid maintenance. , 1986, Plasmid.

[6]  C. Smith,et al.  Regulation of the trfA and trfB promoters of broad host range plasmid RK2: identification of sequences essential for regulation by trfB/korA/korD. , 1985, Nucleic acids research.

[7]  D. Helinski,et al.  Regions of broad-host-range plasmid RK2 involved in replication and stable maintenance in nine species of gram-negative bacteria , 1985, Journal of bacteriology.

[8]  D. Bechhofer,et al.  Replication control in promiscuous plasmid RK2: kil and kor functions affect expression of the essential replication gene trfA , 1985, Journal of bacteriology.

[9]  Christopher M Thomas.  Genetic evidence for the direction of transcription of the trfA gene of broad host range plasmid RK2. , 1984, Journal of general microbiology.

[10]  Christopher M Thomas,et al.  The korB gene of broad host range plasmid RK2 is a major copy number control element which may act together with trfB by limiting trfA expression. , 1984, The EMBO journal.

[11]  Christopher M Thomas,et al.  Analysis of the trfA region of broad host-range plasmid RK2 by transposon mutagenesis and identification of polypeptide products. , 1984, Journal of molecular biology.

[12]  Christopher M Thomas,et al.  Nucleotide sequence of the trfA gene of broad host-range plasmid RK2. , 1984, Journal of molecular biology.

[13]  Christopher M Thomas,et al.  The trfA and trfB promoter regions of broad host range plasmid RK2 share common potential regulatory sequences. , 1984, Nucleic acids research.

[14]  Christopher M Thomas,et al.  Analysis of copy number control elements in the region of the vegetative replication origin of the broad host range plasmid RK2. , 1984, The EMBO journal.

[15]  D. Helinski,et al.  Replication of derivatives of the broad host range plasmid RK2 in two distantly related bacteria. , 1983, Plasmid.

[16]  V. Krishnapillai,et al.  Tn7 insertion mutations affecting the host range of the promiscuous IncP-1 plasmid R18. , 1982, Plasmid.

[17]  Christopher M Thomas,et al.  Maintenance of broad host range plasmid RK2 replicons in Pseudomonas aeruginosa , 1982, Nature.

[18]  Christopher M Thomas.  Complementation analysis of replication and maintenance functions of broad host range plasmids RK2 and RP1. , 1981, Plasmid.

[19]  Christopher M Thomas,et al.  Regions of broad-host-range plasmid RK2 which are essential for replication and maintenance , 1980, Journal of bacteriology.

[20]  H. Birnboim,et al.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA. , 1979, Nucleic acids research.

[21]  Christopher M Thomas,et al.  Incompatibility group P plasmids: genetics, evolution, and use in genetic manipulation. , 1987, Annual review of microbiology.

[22]  R. Kolter,et al.  Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2. , 1979, Methods in enzymology.