Retinyl palmitate–loaded poly(lactide-co-glycolide) nanoparticles for the topical treatment of skin diseases

Nanoparticles were prepared with poly(lactide-co-glycolide), Pluronic F127, and phospholipids and loaded with retinyl palmitate. Morphology and physicochemical properties of these nanoparticles were determined by atomic force microscopy, light scattering, and zeta potential. The elasticity and deformability of the nanoparticles were correlated to Tg values measured by differential scanning calorimetry. The in vitro cytotoxicity and genotoxicity of the nanosystems were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, cell membrane asymmetry, and Ames tests with BALB/3T3 mouse embryo fibroblasts and HaCaT human keratinocytes cell lines. The reactive oxygen species levels and cytokine production in response to the exposure of cells to these nanoparticles were investigated, as well as the penetration in human skin culture.

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