Sol–gel based thermal biosensor for glucose

Abstract A sol–gel (SG) based glucose biosensor using thermometric measurement is reported. The enzymes (glucose oxidase, GOD and catalase, CAT) were entrapped on the surface of reticulated vitreous carbon cylinder (RVC cartridge) using SG as a binder. This ‘RVC cartridge’ was placed within the column of an enzyme thermistor (ET) device. Injection of various d -glucose concentrations resulted in changing the heat content of the circulating buffer, recorded as a thermometric peak by a sensitive thermistor. Independent calibration curves between 10 and 50 mM and between 0.2 and 1 mM d -glucose was obtained by plotting the d -glucose concentration versus the thermometric peak height. The sensitivity of the response was optimized to 1 ml min−1 flow rate of the buffer. The stability of the entrapped GOD/CAT stored at room temperature (25°C) or 4–10°C was 3 or 6 months, respectively. The effect of dissolved oxygen and other interferents such as acetaminophen, ascorbic acid, aspartic acid, glutamic acid, urea and uric acid, on the catalytic activity of the enzyme was also investigated. This system was employed to detect glucose in samples of fruit juice, coca cola and human blood serum.

[1]  C. Brinker,et al.  Sol-Gel Science: The Physics and Chemistry of Sol-Gel Processing , 1990 .

[2]  Somnath Chandra,et al.  Glucose biosensor based on a sol-gel-derived platform , 1994 .

[3]  Modeeparampil N. Kamalasanan,et al.  Electrochemical growth of polyaniline in porous sol-gel films , 1996 .

[4]  S. Sampath,et al.  Sol−Gel Materials in Electrochemistry , 1997 .

[5]  David Avnir,et al.  Enzymes and Other Proteins Entrapped in Sol-Gel Materials , 1994 .

[6]  K. Svanberg,et al.  Coulometric determination of NAD+ and NADH in normal and cancer cells using LDH, RVC and a polymer mediator. , 1999, Talanta.

[7]  Per-Olof Larsson,et al.  Ferrocene-mediated thermal biosensor , 1993 .

[8]  Joseph Wang,et al.  Reticulated vitreous carbon—a new versatile electrode material , 1981 .

[9]  O. Lev,et al.  Sol-gel derived, ferrocenyl-modified silicate-graphite composite electrode: Wiring of glucose oxidase , 1996 .

[10]  K. Ramanathan,et al.  Sol-Gel Derived Ormosil-Exfoliated Graphite−TiO2 Composite Floating Catalyst: Photodeposition of Copper , 1997 .

[11]  J S Valentine,et al.  Encapsulation of proteins in transparent porous silicate glasses prepared by the sol-gel method. , 1992, Science.

[12]  G. Whitesides,et al.  Fabrication of three‐dimensional micro‐structures: Microtransfer molding , 1996 .

[13]  H. Weetall,et al.  Trypsin and Papain Covalently Coupled to Porous Glass: Preparation and Characterization , 1969, Science.

[14]  K R Rogers,et al.  Sol-gel-derived thick-film amperometric immunosensors. , 1998, Analytical chemistry.

[15]  S. Sampath,et al.  Membrane-free, rhodium-modified, methyl silicate-graphite amperometric biosensor , 1997 .

[16]  Stig Bengmark,et al.  CCD-camera based capillary chemiluminescent detection of retinol binding protein , 1999 .

[17]  B. Danielsson,et al.  [The enzyme thermistor]. , 1985, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme.

[18]  D. Avnir,et al.  Simple Absorption Optical Fiber pH Sensor Based on Doped Sol−Gel Cladding Material , 1997 .

[19]  K. Marx,et al.  Sol-Gel Encapsulated Light-Transducing Protein Phycoerythrin: A New Biomaterial , 1995 .

[20]  S. Sampath,et al.  Renewable, reagentless glucose sensor based on a redox modified enzyme and carbon‐silica composite , 1996 .

[21]  K. Nakanishi,et al.  Effect of skeleton size on the performance of octadecylsilylated continuous porous silica columns in reversed-phase liquid chromatography , 1997 .

[22]  David Avnir,et al.  Biochemically active sol-gel glasses: The trapping of enzymes ☆ , 1990 .