A simple method for purification of Strongyloides venezuelensis eggs from rat faeces.

The aim of this study was to develop a simple method to purify Strongyloides eggs from rat faeces using a sucrose gradient centrifugal-flotation technique. This procedure is simple, rapid and possesses a high efficiency in recovering Strongyloides eggs without faecal detritus in less than one hour, thus eliminating the use of complex apparatus and different chemical substances. The possibility of working with pure and live Strongyloides eggs opens up a wide range of future studies on the biology of this parasite. This study constitutes the first report in the scientific literature on purifying Strongyloides eggs using a sucrose density gradient.

[1]  Sang Kyun Park,et al.  An advanced protocol for the purification of whipworm eggs from feces for use as therapeutic agents. , 2019, Parasitology international.

[2]  T. Kikuchi,et al.  Secretome analysis of Strongyloides venezuelensis parasitic stages reveals that soluble and insoluble proteins are involved in its parasitism , 2019, Parasites & Vectors.

[3]  M. K. Nielsen,et al.  Determination of the specific gravity of eggs of equine strongylids, Parascaris spp., and Anoplocephala perfoliata. , 2018, Veterinary parasitology.

[4]  T. Kikuchi,et al.  Comparative transcriptomics gives insights into the evolution of parasitism in Strongyloides nematodes at the genus, subclade and species level , 2018, Scientific Reports.

[5]  Isheng. J. Tsai,et al.  The genome of Strongyloides spp. gives insights into protein families with a putative role in nematode parasitism , 2016, Parasitology.

[6]  T. Kikuchi,et al.  Strongyloides ratti and S. venezuelensis – rodent models of Strongyloides infection , 2016, Parasitology.

[7]  J. Costa-Cruz,et al.  Immunoblotting using Strongyloides venezuelensis larvae, parthenogenetic females or eggs extracts for the diagnosis of experimentally infected immunosuppressed rats. , 2015, Experimental parasitology.

[8]  T. Kikuchi,et al.  Karyotype and reproduction mode of the rodent parasite Strongyloides venezuelensis , 2014, Parasitology.

[9]  P. Payne,et al.  American Association of Veterinary Parasitologists' review of veterinary fecal flotation methods and factors influencing their accuracy and use--is there really one best technique? , 2014, Veterinary parasitology.

[10]  M. Mitreva,et al.  Life Cycle Stage-resolved Proteomic Analysis of the Excretome/Secretome from Strongyloides ratti—Identification of Stage-specific Proteases , 2011, Molecular & Cellular Proteomics.

[11]  J. Utzinger,et al.  Ancylostoma caninum: calibration and comparison of diagnostic accuracy of flotation in tube, McMaster and FLOTAC in faecal samples of dogs. , 2011, Experimental parasitology.

[12]  J. Lok.  Strongyloides stercoralis: a model for translational research on parasitic nematode biology. , 2007, WormBook : the online review of C. elegans biology.

[13]  M. Dryden,et al.  Comparison of common fecal flotation techniques for the recovery of parasite eggs and oocysts. , 2005, Veterinary therapeutics : research in applied veterinary medicine.

[14]  Matilde Galván,et al.  Preliminary testing of a rapid coupled methodology for quantitation/viability determination of helminth eggs in raw and treated wastewater. , 2003, Water research.

[15]  E. D. David,et al.  Determination of the specific gravity of certain helminth eggs using sucrose density gradient centrifugation. , 1982, The Journal of parasitology.

[16]  N. Schaad,et al.  The Use of Density-Gradient Centrifugation for the Purification of Eggs of Meloidogyne spp. , 1975, Journal of nematology.

[17]  M. McClure,et al.  A method for obtaining quantities of clean meloidogyne eggs. , 1973, Journal of nematology.

[18]  R. Beer.  A rapid technique for the concentration and collection of Helminth eggs from large quantities of faeces , 1972, Parasitology.

[19]  D. Denham,et al.  A method for the recovery of nematode eggs from faeces , 1969, Parasitology.