Biological activity of bullfrog growth hormone in the rat and the bullfrog (Rana catesbeiana).

Highly purified bullfrog growth hormone (GH) was tested for growth promoting activity in rats and bullfrogs (Rana catesbeiana). In repeated assays, the potency of bullfrog GH in the rat tibia assay was 1 X bovine GH, which is significantly greater than all other non-mammalian GHs tested. It also stimulated significant whole body growth in immature hypophysectomized rats injected daily for 16 days. In bullfrogs, the bullfrog GH (about 2 microgram/injection) stimulated growth and appetite in intact frogs, and improved survival in hypophysectomized frogs. In contrast, even relatively high doses of bovine GH (up to 200 microgram/injection) were inactive in the bullfrog.

[1]  S. Farmer,et al.  Purification and properties of reptilian and amphibian growth hormones. , 1976, Endocrinology.

[2]  H. Bern,et al.  Purification and properties of teleost growth hormone. , 1976, General and comparative endocrinology.

[3]  P. Licht,et al.  Evolutionary biology of prolactins and somatotropins. II. Electrophoretic comparison of tetrapod somatotropins. , 1971, General and comparative endocrinology.

[4]  S. C. Brown,et al.  Growth and metabolic effects of prolactin and growth hormone in the red-spotted newt, Notophthalmus viridescens. , 1971, The Journal of experimental zoology.

[5]  T. Hayashida,et al.  Immunological studies with rat pituitary growth hormone (RGH). II. Comparative immunochemical investigation of GH from representatives of various vertebrate classes with monkey antiserum to RGH. , 1970, General and comparative endocrinology.

[6]  H. Bern,et al.  Comparative effects of mammalian prolactin and growth hormone on growth in the toads Bufo boreas and Bufo marinus. , 1969, General and Comparative Endocrinology.

[7]  P. S. Brown,et al.  Effects of hypophysectomy, prolactin, and growth hormone on growth of postmetamorphic frogs. , 1969, General and comparative endocrinology.

[8]  N. Moudgal,et al.  Production of antibodies to human pituitary growth hormone in the rat. , 1961, Endocrinology.

[9]  Choh Hao Li,et al.  Difference in Biological Behavior Between Primate and Beef or Whale Pituitary Growth Hormones.∗ , 1959, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[10]  C. Li.  A simplified procedure for the isolation of hypophyseal growth hormone. , 1954, The Journal of biological chemistry.

[11]  F. Greenspan,et al.  Bioassay of hypophyseal growth hormone; the tibia test. , 1949, Endocrinology.