Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor.

A new class of surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) biosensor has been developed for the simultaneous detection of dual DNA markers. The LFA strip in this sensor was composed of two test lines and one control line. SERS nano tags labeled with detection DNA probes were used for quantitative evaluation of dual DNA markers with high sensitivity. Target DNA, associated with Kaposi's sarcoma-associated herpesvirus (KSHV) and bacillary angiomatosis (BA), were tested to validate the detection capability of this SERS-based LFA strip. Characteristic peak intensities of SERS nano tags on two test lines were used for quantitative evaluations of KSHV and BA. The limits of detection for KSHV and BA, determined from our SERS-based LFA sensing platform, were estimated to be 0.043 and 0.074 pM, respectively. These values indicate approximately 10 000 times higher sensitivity than previously reported values using the aggregation-based colorimetric method. We believe that this is the first report of simultaneous detection of two different DNA mixtures using a SERS-based LFA platform. This novel detection technique is also a promising multiplex DNA sensing platform for early disease diagnosis.

[1]  Annalisa D'Andrea,et al.  Lateral flow assay with near-infrared dye for multiplex detection. , 2013, Clinical chemistry.

[2]  Lingxin Chen,et al.  A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA. , 2016, Biosensors & bioelectronics.

[3]  R. Gallo.  The Enigmas of Kaposi's Sarcoma , 1998, Science.

[4]  Chad A Mirkin,et al.  Maximizing DNA loading on a range of gold nanoparticle sizes. , 2006, Analytical chemistry.

[5]  Ye Xu,et al.  Fluorescent probe-based lateral flow assay for multiplex nucleic acid detection. , 2014, Analytical chemistry.

[6]  D. Pang,et al.  Sensitive and Quantitative Detection of C-Reaction Protein Based on Immunofluorescent Nanospheres Coupled with Lateral Flow Test Strip. , 2016, Analytical chemistry.

[7]  E. Cesarman,et al.  Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma. , 1994, Science.

[8]  K. Carron,et al.  Lab-on-a-bubble surface enhanced Raman indirect immunoassay for cholera. , 2012, Analytical chemistry.

[9]  Xia Hu,et al.  Signal enhancement in a lateral flow immunoassay based on dual gold nanoparticle conjugates. , 2013, Clinical biochemistry.

[10]  Neus G Bastús,et al.  Kinetically controlled seeded growth synthesis of citrate-stabilized gold nanoparticles of up to 200 nm: size focusing versus Ostwald ripening. , 2011, Langmuir : the ACS journal of surfaces and colloids.

[11]  A. Berlina,et al.  'Traffic light' immunochromatographic test based on multicolor quantum dots for the simultaneous detection of several antibiotics in milk. , 2015, Biosensors & bioelectronics.

[12]  Molly M Stevens,et al.  Multivalent nanoparticle networks enable point-of-care detection of human phospholipase-A2 in serum. , 2015, ACS nano.

[13]  J. Choo,et al.  Application of a SERS-based lateral flow immunoassay strip for the rapid and sensitive detection of staphylococcal enterotoxin B. , 2016, Nanoscale.

[14]  L. E. De Las Casas,et al.  AIDS presenting with cutaneous Kaposi's sarcoma and bacillary angiomatosis in the bone marrow mimicking Kaposi's sarcoma. , 2002, AIDS patient care and STDs.

[15]  Sang Jun Sim,et al.  Multiplex diagnosis of viral infectious diseases (AIDS, hepatitis C, and hepatitis A) based on point of care lateral flow assay using engineered proteinticles. , 2015, Biosensors & bioelectronics.

[16]  Eun Kyu Lee,et al.  Fabrication of SERS-fluorescence dual modal nanoprobes and application to multiplex cancer cell imaging. , 2012, Nanoscale.

[17]  David Erickson,et al.  Multiplexed colorimetric detection of Kaposi's sarcoma associated herpesvirus and Bartonella DNA using gold and silver nanoparticles. , 2013, Nanoscale.

[18]  Min-Gon Kim,et al.  A three-line lateral flow assay strip for the measurement of C-reactive protein covering a broad physiological concentration range in human sera. , 2014, Biosensors & bioelectronics.

[19]  Bing Yan,et al.  SERS tags: novel optical nanoprobes for bioanalysis. , 2013, Chemical reviews.

[20]  B. Young,et al.  Kaposi's sarcoma versus bacillary angiomatosis. , 1996, Journal of the American Podiatric Medical Association.

[21]  A. Jemal,et al.  Global Cancer Statistics , 2011 .

[22]  A. Jemal,et al.  Trends in Kaposi's sarcoma and non-Hodgkin's lymphoma incidence in the United States from 1973 through 1998. , 2002, Journal of the National Cancer Institute.

[23]  J. Tappero,et al.  Bacillary Angiomatosis or Kaposi's Sarcoma? , 1997 .

[24]  Wang Chunying,et al.  Simultaneous quantitative detection of multiple tumor markers with a rapid and sensitive multicolor quantum dots based immunochromatographic test strip. , 2015 .

[25]  Lisa Lafleur,et al.  Progress toward multiplexed sample-to-result detection in low resource settings using microfluidic immunoassay cards. , 2012, Lab on a chip.

[26]  Rebecca Richards-Kortum,et al.  Multiplexed Recombinase Polymerase Amplification Assay To Detect Intestinal Protozoa. , 2016, Analytical chemistry.

[27]  Eun Kyu Lee,et al.  SERS-based competitive immunoassay of troponin I and CK-MB markers for early diagnosis of acute myocardial infarction. , 2014, Chemical communications.

[28]  Peter B. Luppa,et al.  Point-of-care testing (POCT): Current techniques and future perspectives , 2011, TrAC Trends in Analytical Chemistry.

[29]  E. Bahadır,et al.  Lateral flow assays: Principles, designs and labels , 2016 .

[30]  Il-Hoon Cho,et al.  Plastic ELISA-on-a-chip based on sequential cross-flow chromatography. , 2006, Analytical chemistry.

[31]  Thomas L. Madden,et al.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. , 1997, Nucleic acids research.

[32]  Bong Hyun Chung,et al.  Rapid and sensitive phenotypic marker detection on breast cancer cells using surface-enhanced Raman scattering (SERS) imaging. , 2014, Biosensors & bioelectronics.