Electromagnetic fields enhance the stress response at elevated temperatures in the nematode Caenorhabditis elegans.

We have studied the effect of extremely low frequency electromagnetic fields (ELF-EMF) in the presence of a second stressor (mild heat shock) on the expression of a lacZ reporter gene under the control of hsp16 or hsp70 promoters in two transgenic strains of C. elegans. The expression of the reporter gene was studied by scoring animals with induced beta-galactosidase activity after staining in toto or by biochemical quantitation of the enzyme activity, respectively. In our experimental setup we were able to expose the animals to 50 Hz magnetic flux density of 0-150 microT and at the same time control temperature with high precision (+/-0.1 degrees C). Experimental conditions were defined for which EMF strongly enhances the expression of the reporter gene.

[1]  R. Goodman,et al.  Magnetic field stress induces expression of hsp70. , 1998, Cell stress & chaperones.

[2]  K. Lowe,et al.  Effects of metal ions in combination with a non-ionic surfactant on stress responses in a transgenic nematode , 1997 .

[3]  M. Kogevinas,et al.  Need for a European approach to the effects of extremely low-frequency electromagnetic fields on cancer. ELF-EMF European Feasibility Study Group. , 1997, Scandinavian Journal of Work, Environment and Health.

[4]  R. Stevens,et al.  The melatonin hypothesis: electric power and breast cancer. , 1996, Environmental health perspectives.

[5]  E. Candido,et al.  Expression of the polyubiquitin-encoding gene (ubq-1) in transgenic Caenorhabditis elegans. , 1992, Gene.

[6]  E. Candido,et al.  Transgenic strains of the nematode C. elegans in biomonitoring and toxicology: effects of captan and related compounds on the stress response. , 1996, Toxicology.

[7]  D. D. Pomerai,et al.  Evaluation of a stress-inducible transgenic nematode strain for rapid aquatic toxicity testing , 1994 .

[8]  J. Kirschvink,et al.  Uniform magnetic fields and double-wrapped coil systems: improved techniques for the design of bioelectromagnetic experiments. , 1992, Bioelectromagnetics.

[9]  R. Hesketh,et al.  Biological responses to electromagnetic fields 1 , 1998, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[10]  D. D. de Pomerai,et al.  Use of Stress-Inducible Transgenic Nematodes as Biomarkers of Heavy Metal Pollution in Water Samples from an English River System , 1997, Archives of environmental contamination and toxicology.

[11]  E. Candido,et al.  Transgenic Caenorhabditis elegans strains as biosensors. , 1996, Trends in biotechnology.

[12]  R. Goodman,et al.  Myc‐mediated transactivation of HSP70 expression following exposure to magnetic fields , 1998, Journal of cellular biochemistry.

[13]  Martin Blank,et al.  Increased levels of hsp70 transcripts induced when cells are exposed to low frequency electromagnetic fields , 1994 .

[14]  L. Gaboury,et al.  Evaluation of the potential carcinogenicity of 60 Hz linear sinusoidal continuous‐wave magnetic fields in Fischer F344 rats , 1997, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[15]  R. Goodman,et al.  Electromagnetic field exposure induces rapid, transitory heat shock factor activation in human cells , 1997, Journal of cellular biochemistry.

[16]  S. Brenner The genetics of Caenorhabditis elegans. , 1974, Genetics.

[17]  G. Stroink,et al.  Uniform magnetic field produced by three, four, and five square coils , 1983 .

[18]  R. Kavet,et al.  Can low-level 50/60 Hz electric and magnetic fields cause biological effects? , 1997, Radiation research.

[19]  C. Dees,et al.  Effects of 60-Hz fields, estradiol and xenoestrogens on human breast cancer cells. , 1996, Radiation research.

[20]  W. Löscher,et al.  Exposure of DMBA-treated female rats in a 50-Hz, 50 microTesla magnetic field: effects on mammary tumor growth, melatonin levels, and T lymphocyte activation. , 1996, Carcinogenesis.

[21]  E. Candido,et al.  Transgenic hsp 16‐Lacz strains of the soil nematode caenorhabditis elegans as biological monitors of environmental stress , 1994 .

[22]  J. McCann,et al.  A critical review of the genotoxic potential of electric and magnetic fields. , 1993, Mutation research.

[23]  David Weisbrot,et al.  Transcription in Drosophila melanogaster salivary gland cells is altered following exposure to low‐frequency electromagnetic fields: Analysis of chromosome 3R , 1992 .