The chemical basis of the virulence of Bacillus anthracis. V. The specific toxin produced by B. Anthracis in vivo.

(250 i 25 g.) were injected in the saphenous vein using a local anaesthetic. Volumes greater than 5 ml. were given in two spaced injections. If the volume to be injected was more than 10 ml. for guinea-pigs or 1 ml. for mice, the plasma was freeze-dried, reconstituted and injected at twice the original concentration. All samples were centrifuged to remove gross particles. Results are reported as deaths (numerator) occurring in groups (denominator) of animals during 7 days' observation.