Epidemiology of Porcine Pseudorabies from 2010 to 2018 in Tianjin, China.

Pseudorabies (PR), the causative agent of Aujeszky's disease, has rapidly increased in recent years and has caused significant economic losses. To understand the seroprevalence and epidemiological characteristics of PR in Tianjin, China, a total of 23,627 blood and 1,093 tissue samples were collected from 228 pig farms during January 2010 to December 2018. The Pseudorabies virus (PRV) glycoprotein E (gE) antibody was tested by enzyme-linked immunosorbent assay (ELISA), and wild-type PRV (WT PRV) was detected by gE-polymerase chain reaction (PCR). Macroscopic and microscopic lesions were observed in tissue samples. The results showed that 46.70% of the serum samples and 49.76% of pig farms were seropositive for PRV gE antibody based on the ELISA results, and 13.54% of the tissue samples were positive for WT PRV detected by PCR. The positive rate of serum samples increased rapidly after 2011 and reached 62.40% in 2013. Although it gradually decreased from 2014 to 2018, the positive rate of serum samples remained at a high level. The positive rate of pig farms showed the same trend. Moreover, after 2011, the detection rate of WT PRV was increased rapidly and was significantly higher than in 2010 and 2011. Macroscopic and microscopic lesions were observed in various tissues during histopathological examination. Based on univariate analysis, the increased risk of seropositivity was associated with the immune status and infection in sows and fattening pigs. These findings demonstrate that PR was prevalent in the region of Tianjin, China. These epidemiological data can assist in the control of PR.

[1]  Sidang Liu,et al.  Epidemiological investigation of porcine pseudorabies virus and its coinfection rate in Shandong Province in China from 2015 to 2018 , 2020, Journal of veterinary science.

[2]  Chao Sui,et al.  Characterization of a moderately pathogenic pseudorabies virus variant isolated in China, 2014. , 2019, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[3]  P. Jiang,et al.  Comparative pathogenicity and immunogenicity of triple and double gene-deletion pseudorabies virus vaccine candidates. , 2017, Research in veterinary science.

[4]  Song Gao,et al.  Bartha-k61 vaccine protects growing pigs against challenge with an emerging variant pseudorabies virus. , 2017, Vaccine.

[5]  Sidang Liu,et al.  Seroprevalence and associated risk factors of pseudorabies in Shandong province of China , 2016, Journal of veterinary science.

[6]  M. Chien,et al.  Growth properties and vaccine efficacy of recombinant pseudorabies virus defective in glycoprotein E and thymidine kinase genes. , 2016, Journal of biotechnology.

[7]  Wu Tong,et al.  A live, attenuated pseudorabies virus strain JS-2012 deleted for gE/gI protects against both classical and emerging strains. , 2016, Antiviral research.

[8]  Wu Tong,et al.  Emergence of a Pseudorabies virus variant with increased virulence to piglets. , 2015, Veterinary microbiology.

[9]  M. Shi,et al.  Genomic characterization of emergent pseudorabies virus in China reveals marked sequence divergence: Evidence for the existence of two major genotypes. , 2015, Virology.

[10]  Xiangdong Li,et al.  Construction of a triple gene-deleted Chinese Pseudorabies virus variant and its efficacy study as a vaccine candidate on suckling piglets. , 2015, Vaccine.

[11]  H. Qiu,et al.  Pathogenicity and genomic characterization of a pseudorabies virus variant isolated from Bartha-K61-vaccinated swine population in China. , 2014, Veterinary microbiology.

[12]  H. Qiu,et al.  A novel gE-deleted pseudorabies virus (PRV) provides rapid and complete protection from lethal challenge with the PRV variant emerging in Bartha-K61-vaccinated swine population in China. , 2014, Vaccine.

[13]  G. Tong,et al.  Pseudorabies Virus Variant in Bartha-K61–Vaccinated Pigs, China, 2012 , 2013, Emerging infectious diseases.

[14]  Xuke Zhang,et al.  Emergence of virulent pseudorabies virus infection in Northern China , 2013, Journal of veterinary science.

[15]  M. I. Quiroga,et al.  Pseudorabies virus infection in mink: a host-specific pathogenesis. , 2008, Veterinary immunology and immunopathology.