Chitinolytic activity of actinomycetes from a cerrado soil and their potential in biocontrol

R.C. GOMES, L.T.A.S. SEME(r)DO, R.M.A. SOARES, C.S. ALVIANO, L.F. LINHARES and R.R.R. COELHO.2000.The crude enzyme extracts from five actinomycetes selected from a cerrado soil presented very good endochitinolytic activity when compared to a commercial chitinase. Exochitinase and chitobiase activities were also detected. They were identified as Streptomyces, but could not be characterized to species level, probably corresponding to new ones. The crude extracts, obtained from growth on fungal mycelium plus chitin of three of the strains, have shown a very pronounced activity against phytopathogenic fungi. In tests using growing cells, all five strains were active. These data suggest that these strains are potential biocontrol agents.

[1]  H. Lechevalier,et al.  Chemical composition as a criterion in the classification of aerobic actinomycetes , 1970 .

[2]  N. Cresswell,et al.  Molecular detection of streptomycin-producing streptomycetes in Brazilian soils , 1997, Applied and environmental microbiology.

[3]  I. Chet,et al.  Evidence that chitinase produced by Aeromonas caviae is involved in the biological control of soil-borne plant pathogens by this bacterium , 1991 .

[4]  A. Asselin,et al.  Detection of chitinase activity after polyacrylamide gel electrophoresis. , 1989, Analytical biochemistry.

[5]  M. Deshpande,et al.  CHITINASE PRODUCTION BY MYROTHECIUM VERRUCARIA AND ITS SIGNIFICANCE FOR FUNGAL MYCELIA DEGRADATION , 1989 .

[6]  Y. Elad,et al.  DEGRADATION OF PLANT PATHOGENIC FUNGI BY TRICHODERMA HARZIANUM , 1982 .

[7]  P. Sneath,et al.  Numerical classification of Streptomyces and related genera. , 1983, Journal of general microbiology.

[8]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[9]  R. Coelho,et al.  Efficiency of the dispersion and differential centrifugation technique in the isolation of chitinolytic actinomycetes from soil , 1999 .

[10]  E. Cabib,et al.  A rapid and sensitive assay for chitinase using tritiated chitin. , 1977, Analytical biochemistry.

[11]  C. Kubicek,et al.  Parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action of Trichoderma harzianum against phytopathogenic fungi , 1994, Applied and environmental microbiology.

[12]  M. G. Ferri,et al.  Ecologia do cerrado , 1979 .

[13]  T. Wood,et al.  METHODS FOR MEASURING CELLULASE ACTIVITIES , 1988 .

[14]  J. S. Virdi,et al.  Chitinase production by Streptomyces viridificans: its potential in fungal cell wall lysis. , 1995, The Journal of applied bacteriology.

[15]  J. Lockwood,et al.  Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. , 1975, Applied microbiology.

[16]  M. Sela-Buurlage,et al.  Only Specific Tobacco (Nicotiana tabacum) Chitinases and [beta]-1,3-Glucanases Exhibit Antifungal Activity , 1993, Plant physiology.

[17]  Yigal Elad,et al.  The role of chitinase of Serratia marcescens in biocontrol of Sclerotium rolfsii , 1988 .

[18]  M. M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[19]  A. Bull,et al.  Biodiversity as a source of innovation in biotechnology. , 1992, Annual review of microbiology.

[20]  L. Selbmann,et al.  Chitinolytic activity at low temperature of an Antarctic strain (A3) of Verticillium lecanii. , 1998, Research in microbiology.

[21]  W. K. Roberts,et al.  PLANT AND BACTERIAL CHITINASES DIFFER IN ANTIFUNGAL ACTIVITY , 1988 .

[22]  J. Lynch,et al.  Isolation and Characterization of Actinomycete Antagonists of a Fungal Root Pathogen , 1993, Applied and environmental microbiology.