In vitro platelet adhesion to vascular subendothelium: Studies in patients with polycythaemia vera

In this work we studied platelet adhesion to subendothelial surfaces in 10 patients with polycythaemia vera and 10 healthy volunteers at 40% Hct (corresponding to the mean value of our control group) and 55% Hct (a value roughly corresponding to the mean Hct in polycythaemic patients). Platelet concentration was kept constant at 2.0–2.5 × 1011/l. The results indicate that there was a statistically significant increase in adhesion both in controls and in patients with Hct varying from 40% to 55%. The contribution of the higher Hct in promoting platelet adhesion was comparable in the two groups. When red blood cells (RBC) from the patients were tested with platelets from healthy volunteers in cross‐over experiments, they promoted adhesion in the same way as control RBC. Similarly, when patients' platelets were mixed with control RBC, adhesion was the same as control platelets. These data indicate that platelet and RBC contribution to this parameter are not significantly modified in this group of polycythaemic patients, provided that platelet and RBC values are adjusted to control range.

[1]  M. Rampling,et al.  A study of the haematological and haemorheological consequences of venesection , 1986, British journal of haematology.

[2]  T. Barbui,et al.  Platelet 5-hydroxytryptamine transport and storage in myeloproliferative disorders. , 2009, Scandinavian journal of haematology.

[3]  P. Clézardin,et al.  Platelet membrane glycoprotein abnormalities in patients with myeloproliferative disorders and secondary thrombocytosis , 1985, British journal of haematology.

[4]  E. Dejana,et al.  Human platelet adhesion to subendothelium under controlled hemodynamic conditions: a methodological approach. , 1985, Methods and findings in experimental and clinical pharmacology.

[5]  R. Heethaar,et al.  Red blood cell deformability influences platelets--vessel wall interaction in flowing blood. , 1984, Blood.

[6]  A. Schafer.  Bleeding and thrombosis in the myeloproliferative disorders. , 1984, Blood.

[7]  I. Tatarsky,et al.  Moving boundary electrophoresis of red cells on differently treated polycythaemic patients. , 2009, Scandinavian journal of haematology.

[8]  R. Heethaar,et al.  Red blood cell size is important for adherence of blood platelets to artery subendothelium , 1983 .

[9]  S. Jain,et al.  Red cell membrane stiffness in iron deficiency. , 1983, Blood.

[10]  M. Greaves,et al.  Evidence for a platelet membrane defect in the myeloproliferative syndromes. , 1982, Thrombosis research.

[11]  P. Mannucci,et al.  Biochemical and Metabolic Aspects of Platelet Dysfunction in Chronic Myeloproliferative Disorders , 1982, Thrombosis and Haemostasis.

[12]  B. Boneu,et al.  Platelets in myeloproliferative disorders. III: Glycoprotein profile in relation to platelet function and platelet density. , 2009, Scandinavian journal of haematology.

[13]  G. Mascaro,et al.  Defective platelet aggregation and increased platelet turnover in patients with myelofibrosis and other myeloproliferative diseases. , 2009, Scandinavian journal of haematology.

[14]  Brown Ja,et al.  Abnormal platelet function in myeloproliferative disorders. , 1981 .

[15]  A. Marmur,et al.  Moving Boundary Electrophoresis and Sialic Acid Content of Normal and Polycythaemic Red Blood Cells , 1981, British journal of haematology.

[16]  E. Rewald,et al.  DELAY IN RED CELL FILTRATION IN POLYCYTHAEMIC PATIENTS , 1981, British journal of haematology.

[17]  J. A. Brown,et al.  Abnormal platelet function in myeloproliferative disorders. , 1981, Archives of pathology & laboratory medicine.

[18]  G. Laurent,et al.  Platelets in myeloproliferative disorders. II. Serotonin uptake and storage: correlations with mepacrine labelled dense bodies and with platelet density. , 2009, Scandinavian journal of haematology.

[19]  G. Laurent,et al.  Platelets in myeloproliferative disorders. I. A comparative evaluation with certain platelet function tests. , 2009, Scandinavian journal of haematology.

[20]  H. Weiss,et al.  Red blood cells: their dual role in thrombus formation. , 1980, Science.

[21]  Cantab Mb.  Christopher George Attenborough. , 1979 .

[22]  J. Cazenave,et al.  Quantitative radioisotopic measurement and scanning electron microscopic study of platelet adherence to a collagen-coated surface and to subendothelium with a rotating probe device. , 1979, The Journal of laboratory and clinical medicine.

[23]  T. Pearson,et al.  VASCULAR OCCLUSIVE EPISODES AND VENOUS HÆMATOCRIT IN PRIMARY PROLIFERATIVE POLYCYTHÆMLX , 1978, The Lancet.

[24]  T. Pearson,et al.  Vascular occlusive episodes and venous haematocrit in primary proliferative polycythaemia. , 1978, Lancet.

[25]  T. Pearson,et al.  EFFECT OF HÆMATOCRIT ON CEREBRAL BLOOD-FLOW IN MAN , 1977, The Lancet.

[26]  R. Russell,et al.  CEREBRAL BLOOD-FLOW IN POLYCYTHÆMIA , 1977, The Lancet.

[27]  R. Russell,et al.  Effect of haematocrit on cerebral blood-flow in man. , 1977, Lancet.

[28]  J. Whittembury,et al.  Cerebral blood-flow in polycythaemia. , 1977, Lancet.

[29]  Lond Mb.  Frederick Ronald Chistopher Casson. , 1975 .

[30]  N. Berlin.  Diagnosis and classification of the polycythemias. , 1975, Seminars in hematology.

[31]  L. Wasserman,et al.  Abnormalities of platelet function in patients with polycythemia vera. , 1973, Cancer research.

[32]  J. Mustard,et al.  Preparation of Suspensions of Washed Platelets from Humans , 1972, British journal of haematology.

[33]  T. Thiede,et al.  Complications and causes of death in polycythaemia vera. , 2009, Acta medica Scandinavica.

[34]  L. Lorand,et al.  Studies on apyrases. , 1961, Archives of biochemistry and biophysics.