Effect of washing on the bioefficacy of insecticide-treated nets (ITNs) and long-lasting insecticidal nets (LLINs) against main malaria vector Anopheles stephensi by three bioassay methods.

BACKGROUND & OBJECTIVES The use of pyrethoid impregnated bednets is one of the main malaria vector control strategies worldwide. The objective of the present study was to evaluate the bioefficacy of bednets impregnated with various pyrethroids after repeated washings. METHODS The effectiveness of bednets impregnated with permethrin, deltamethrin, bifenthrin, etofenprox and long-lasting bednets like OlysetNet and PermaNet which were provided by WHOPES was evaluated. The tests were carried out according to the WHO-recommended methods. Malaria vector, Anopheles stephensi was exposed to impregnated bednets for 3 min and the mortality was measured after 24 h recovery period. Knockdown was measured as well. RESULTS Results of three methods of bioassay tests showed that between two LLINs, PermaNet was more efficient than OlysetNet. Results of ITNs exhibited that deltamethrin and permethrin were more effective than etofenprox and bifenthrin as impregnants. INTERPRETATION & CONCLUSION Findings of this study will be useful for WHO, local authorities and people who wish to use different pyrethroid-impregnated bednets for malaria vector control.

[1]  M. A. Ansari,et al.  EFFICACY OF PERMANET® 2.0 AGAINST ANOPHELES CULICIFACIES AND ANOPHELES STEPHENSI, MALARIA VECTORS IN INDIA , 2007, Journal of the American Mosquito Control Association.

[2]  A. Dash,et al.  Wash Resistance and Bioefficacy of Olyset Net — A Long-Lasting Insecticide-Treated Mosquito Net Against Malaria Vectors and Nontarget Household Pests , 2006, Journal of medical entomology.

[3]  M. A. Ansari,et al.  BIOEFFICACY OF OLYSET® NETS AGAINST MOSQUITOES IN INDIA , 2006, Journal of the American Mosquito Control Association.

[4]  M. Rowland,et al.  Multi‐country field trials comparing wash‐resistance of PermaNet™ and conventional insecticide‐treated nets against anopheline and culicine mosquitoes , 2005, Medical and veterinary entomology.

[5]  T. Adak,et al.  PERSISTENCE AND WASH-RESISTANCE OF INSECTICIDAL EFFICACY OF NETTINGS TREATED WITH DELTAMETHRIN TABLET FORMULATION (K-O TAB®) AGAINST MALARIA VECTORS , 2005, Journal of the American Mosquito Control Association.

[6]  S. Frances,et al.  Comparative toxicity of permethrin- and bifenthrin-treated cloth fabric for Anopheles farauti and Aedes aegypti. , 2003, Journal of the American Mosquito Control Association.

[7]  C. Rogier,et al.  Comparative performances, under laboratory conditions, of seven pyrethroid insecticides used for impregnation of mosquito nets. , 2003, Bulletin of the World Health Organization.

[8]  W. Takken Do insecticide‐treated bednets have an effect on malaria vectors? , 2002, Tropical medicine & international health : TM & IH.

[9]  F. Chandre,et al.  Insecticide mixtures for mosquito net impregnation against malaria vectors. , 2002, Parasite.

[10]  A. Kroeger,et al.  Wash resistance of insecticide-treated materials. , 2002, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[11]  A. Aitio,et al.  Safety of pyrethroid‐treated mosquito nets , 2000, Medical and veterinary entomology.

[12]  C. Prasittisuk,et al.  The effect of pyrethroid impregnated mosquito nets on field malaria vector populations in experimental huts and in individual local houses. , 1996, The Southeast Asian journal of tropical medicine and public health.

[13]  S. Teutsch,et al.  The effectiveness of insecticide-impregnated bed nets in reducing cases of malaria infection: a meta-analysis of published results. , 1995, The American journal of tropical medicine and hygiene.

[14]  J. Lines,et al.  Book reviewA global strategy for malaria control: Geneva: World Health Organization, 1993. x + 30pp. Price Sw. fr. 11/US $9.90 (in developing countries Sw. fr. 7.70). ISBN 92-4-156161-0☆ , 1994 .

[15]  S. Lindsay,et al.  Experimental hut trials of bednets impregnated with synthetic pyrethroid or organophosphate insecticide for mosquito control in The Gambia , 1991, Medical and veterinary entomology.

[16]  H. Yap,et al.  Chemical Methods for the Control of Vectors and Pests of Public Health Importance , 1997 .