In vivo function of hemolysin in the nephropathogenicity of Escherichia coli

The role of hemolysin in the nephropathogenicity of Escherichia coli was studied in a hematogenous pyelonephritis model in mice. The nephropathogenicity of a nonhemolytic, avirulent E. coli strain was increased by simultaneous injection with its hemolytic, nephropathogenic parent. This helper mechanism could be attributed to hemolysin, since the simultaneous injection of partially purified hemolysin gave a similar enhancement of nephropathogenicity. Intraperitoneal injection of hemoglobin or iron sulfate before intravenous challenge with this avirulent strain also led to increased virulence. The nephropathogenicity-enhancing effect of hemolysin is therefore supposed to depend on increasing the level of available iron in the host. Under conditions of plentiful iron, hemolysin production was repressed, as shown by in vitro growth experiments in the presence of exogenous iron. These results suggest that the production of hemolysin is regulated by feedback inhibition.

[1]  C. Waalwijk,et al.  Effect of the pH on the hemolytic titer of Escherichia coli strains , 1983 .

[2]  I. Snyder,et al.  Effect of Escherichia coli alpha-hemolysin on human peripheral leukocyte function in vitro , 1982, Infection and Immunity.

[3]  R. Glassock,et al.  Effect of iron on acute pyelonephritis and later chronic changes. , 1982, Kidney international.

[4]  I. Snyder,et al.  Effect of Escherichia coli alpha-hemolysin on human peripheral leukocyte viability in vitro , 1982, Infection and immunity.

[5]  P. Brandt,et al.  Haptoglobin: a natural bacteriostat. , 1982, Science.

[6]  M. A. Linggood,et al.  The role of alpha haemolysin in the virulence of Escherichia coli for mice. , 1982, Journal of medical microbiology.

[7]  F. Baglia,et al.  Haptoglobin biosynthesis in rats. Immunological identification of polysomes synthesizing haptoglobin and quantitation of haptoglobin in the cytoplasm of liver cells. , 1982, Biochimica et biophysica acta.

[8]  J. Bullen.  The significance of iron in infection. , 1981, Reviews of infectious diseases.

[9]  J. de Graaff,et al.  Virulence of Escherichia coli in experimental hematogenous pyelonephritis in mice , 1979, Infection and immunity.

[10]  J. Armstrong,et al.  The role of lactoferrin in the bactericidal function of polymorphonuclear leucocytes. , 1979, Immunology.

[11]  R. Simmons,et al.  Mechanisms of the adjuvant effect of hemoglobin in experimental peritonitis. I. In vivo inhibition of peritoneal leukocytosis. , 1978, Surgery.

[12]  R. Simmons,et al.  Mechanisms of the adjuvant action of hemoglobin in experimental peritonitis. 2. Influence of hemoglobin on human leukocyte chemotaxis in vitro. , 1977, The Journal of surgical research.

[13]  P. Quie,et al.  Effect of iron on leukocyte function: inactivation of H2O2 BY IRON , 1975, Infection and immunity.

[14]  E. Weinberg,et al.  Iron and susceptibility to infectious disease. , 1974, Science.

[15]  J. Fletcher.  The effect of iron and transferrin on the killing of Escherichia coli in fresh serum. , 1971, Immunology.

[16]  W. Gill,et al.  Etiology of pyelonephritis. Renal lysosome disruption by hemolytic Escherichia coli. , 1971, Investigative urology.

[17]  G. Gladstone,et al.  Effect of Iron on the Bactericidal Proteins from Rabbit Polymorphonuclear Leucocytes , 1970, Nature.

[18]  E. Goldstein,et al.  The effect of parenteral iron preparations on experimental pyelonephritis. , 1970, British journal of experimental pathology.

[19]  H. Rogers,et al.  The effect of iron compounds on the virulence of Escherichia coli for guinea-pigs. , 1968, Immunology.

[20]  I. Cohn,et al.  Hemoglobin and Escherichia coli, a Lethal Intraperitoneal Combination , 1968, Journal of bacteriology.

[21]  H. Smith.  The haemolysins of Escherichia coli. , 1963, The Journal of pathology and bacteriology.