Western immunoblotting: temperature-dependent reduction in background staining.

[1]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[2]  J. Adams Heavy metal intensification of DAB-based HRP reaction product. , 1981, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[3]  C. Milstein,et al.  [1] Preparation of monoclonal antibodies: Strategies and procedures , 1981 .

[4]  W. N. Burnette,et al.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. , 1981, Analytical biochemistry.

[5]  J. Gershoni,et al.  Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter. , 1982, Analytical biochemistry.

[6]  W. van Dijk,et al.  Specific detection of inactive enzyme protein after polyacrylamide gel electrophoresis by a new enzyme-immunoassay method using unspecific antiserum and partially purified active enzyme: application to rat liver phosphodiesterase I. , 1982, Analytical biochemistry.

[7]  W. Newhall,et al.  The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. , 1982, Journal of immunological methods.

[8]  H. Kasamatsu,et al.  On the electrotransfer of polypeptides from gels to nitrocellulose membranes. , 1983, Analytical biochemistry.

[9]  H. Towbin,et al.  Immunoblotting and dot immunobinding--current status and outlook. , 1984, Journal of immunological methods.

[10]  J. Sportsman,et al.  Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose , 1984 .

[11]  J. Cannon,et al.  Different blocking agents cause variation in the immunologic detection of proteins transferred to nitrocellulose membranes. , 1985, Journal of immunological methods.

[12]  Effects of the blocking agents bovine serum albumin and Tween 20 in different buffers on immunoblotting of brain proteins and marker proteins. , 1986, Journal of immunological methods.

[13]  W. L. Hoffman,et al.  Tween 20 removes antibodies and other proteins from nitrocellulose. , 1986, Journal of immunological methods.

[14]  C. Bird,et al.  The use of Tween 20 alone as a blocking agent for immunoblotting can cause artefactual results. , 1988, Journal of immunological methods.