Matrix-assisted laser desorption ionization mass spectrometry of proteins electroblotted after polyacrylamide gel electrophoresis

Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) of proteins, electroblotted onto polymer membranes after sodium dodecyl sulfate-polyacrylamide gel electropboresis (SDS-PAGE) separation is demonstrated. The proteins are desorbed directly from the blot membranes after matrix application. Desorption with 2.94-μm infrared radiation and succinic acid as matrix was found superior to 355-nm UV desorption using 2,5-dihydroxybenzoic acid as matrix. Several commercially available membranes tested resulted in protein signals after matrix incubation of the membrane. Systematic investigations for five different poly(vinylidene fluoride) (PVDF) membranes showed improved results for membranes exhibiting high specific surfaces

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