AFLP analysis of the critically endangered Limonium cavanillesii (Plumbaginaceae)

Genetic variation in the only remaining population of Limonium cavanillesii has been assayed by means of AFLP analysis. The use of three different primers provided 231 marker bands, 13 of which were polymorphic among the 29 individuals assayed, thus allowing their classification into 11 distinct, but very closely related phenotypes. The low genetic variability found in L. cavanillesii could be explained both by the apomictic reproductive system of this species and by the passage through a severe bottleneck in recent times, after which there has been no chance for mutation to restore higher genetic variation. These results are compared to previous data obtained with RAPDs using the same L. cavanillesii individuals. In this case, AFLPs seem to represent a significantly more variable class of markers than RAPDs, as no variation was found with the later. Consequently a genetic marker is now available for the implementation and monitoring of conservation measures for this critically endangered species.

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