Characterization and use of a penicillin acylase biocatalyst.

A complete characterization of a penicillin acylase biocatalyst is presented, including the determination of physicochemical and kinetic parameters. Stability studies are detailed in terms of both storage temperature and pH as well as operational stability after 150 batch reactions of two hours duration each. An Arrhenius-type model was used to simulate the effect of pH on biocatalyst stability. A kinetic model is proposed to describe batch and continuous stirred tank reactors and to predict the long-term behavior of the process.

[1]  F. Wagner,et al.  Penicillin acylase from the hybrid strains Escherichia coli 5K(pHM12): enzyme formation and hydrolysis of beta-lactam antibiotics with whole cells , 1984, Applied and environmental microbiology.

[2]  R. Crichton,et al.  Stabilisation and immobilisation of penicillin amidase , 1989, FEBS letters.

[3]  A. Trochimczuk,et al.  Immobilization of penicillin acylase on acrylic carriers , 1989 .

[4]  J. Slezák,et al.  Penicillinamidohydrolase in Escherichia coli. II. Synthesis of the enzyme, kinetics and specificity of its induction and the effect of O2. , 1975, Folia microbiologica.

[5]  D. Dennen.  Degradation kinetics of 6-aminopenicillanic acid. , 1967, Journal of pharmaceutical sciences.

[6]  P Dunnill,et al.  The isolation and kinetics of penicillin amidase from Escherichia coli. , 1972, Biochimica et biophysica acta.

[7]  J. Slezák,et al.  Penicillinamidohydrolase in Escherichia coli. III. Catabolite repression, diauxie, effect of cAMP and nature of the enzyme induction. , 1975, Folia microbiologica.

[8]  S. B. Lee,et al.  Reaction kinetics and mechanisms of penicillin amidase: A comparative study by computer simulation , 1982 .

[9]  J. Shewale,et al.  Evaluation of determination of 6-aminopenicillanic acid by p -dimethyl aminobenzaldedyde , 1987 .

[10]  H. Sivaraman,et al.  PENICILLIN ACYLASE: ENZYME PRODUCTION AND ITS APPLICATION IN THE MANUFACTURE OF 6-APA , 1989 .

[11]  Poul Borge Poulsen,et al.  Current Applications of Immobilized Enzymes for Manufacturing Purposes , 1984 .

[12]  J. P. Cardoso,et al.  Modelling of the Hydrolysis of Benzylpenicillin to 6‐Aminopenicillanic Acid and Phenyl Acetic Acid by an Immobilised Penicillin Amidase in a Small Pilot Plant Batch Recirculated Reactor , 1986 .

[13]  Peter Dunnill,et al.  Conversion of benzylpenicillin to 6‐aminopenicillanic acid in a batch reactor and continuous feed stirred tank reactor using immobilized penicillin amidase , 1973 .

[14]  S. W. Carleysmith,et al.  Kinetic behavior of immobilized Penicillin acylase , 1980 .

[15]  M. Shimizu,et al.  Enzymatic synthesis of cphalosporins. III. Purification and properties of penicillin acylase from Kluyvera citrophila. , 1975 .

[16]  C. Kutzbach,et al.  Preparation and General Properties of Crystalline Penicillin Acylase from Escherichia coli ATCC 11 105 , 2009, Hoppe-Seyler's Zeitschrift fur physiologische Chemie.

[17]  K. Burg,et al.  Neue synthetische Träger zur Fixierung von Enzymen , 1988 .

[18]  F. Le Goffic,et al.  The immobilization of penicillin G acylase on chitosan. , 1989, Biotechnology and bioengineering.

[19]  W. Marconi,et al.  The hydrolysis of penicillin G to 6-amino penicillanic acid by entrapped penicillin acylase. , 1973, The Journal of antibiotics.