Harmonic Convergence in the Love Songs of the Dengue Vector Mosquito

The familiar buzz of flying mosquitoes is an important mating signal, with the fundamental frequency of the female's flight tone signaling her presence. In the yellow fever and dengue vector Aedes aegypti, both sexes interact acoustically by shifting their flight tones to match, resulting in a courtship duet. Matching is made not at the fundamental frequency of 400 hertz (female) or 600 hertz (male) but at a shared harmonic of 1200 hertz, which exceeds the previously known upper limit of hearing in mosquitoes. Physiological recordings from Johnston's organ (the mosquito's “ear”) reveal sensitivity up to 2000 hertz, consistent with our observed courtship behavior. These findings revise widely accepted limits of acoustic behavior in mosquitoes.

[1]  A. Mayer.  Experiments on the Supposed Auditory Apparatus of the Mosquito , 1874, The American Naturalist.

[2]  L. Roth,et al.  A Study of Mosquito Behavior. An Experimental Laboratory Study of the Sexual Behavior of Aedes aegypti (Linnaeus) , 1948 .

[3]  M. C. Kahn,et al.  The first field tests of recorded mosquito sounds used for mosquito destruction. , 1949, American Journal of Tropical Medicine and Hygiene.

[4]  E. F. Knipling,et al.  Eradication of Screw-Worms through Release of Sterilized Males. , 1955, Science.

[5]  G. Wishart,et al.  Flight Responses to Various Sounds by Adult Males of Aedes aegypti (L.) (Diptera: Culicidae) , 1959, The Canadian Entomologist.

[6]  M. Rockstein.  The physiology of Insecta , 1964 .

[7]  J. A. Downes.  The Swarming and Mating Flight of Diptera , 1969 .

[8]  L. Barton Browne,et al.  Experimental Analysis of Insect Behaviour , 1974, Springer Berlin Heidelberg.

[9]  K. Boo,et al.  Fine structure of scolopidia in Johnston's organ of female Aedes aegypti compared with that of the male. , 1975, Journal of insect physiology.

[10]  Peter Belton,et al.  FLIGHT SOUNDS OF THE FEMALES OF SOME MOSQUITOES OF WESTERN CANADA , 1979 .

[11]  A. M. Young,et al.  Renewal of sexual receptivity in mated female mosquitoes, Aedes aegypti , 1982 .

[12]  K. Hagino-Yamagishi,et al.  [Oncogene]. , 2019, Gan to kagaku ryoho. Cancer & chemotherapy.

[13]  A. Palmer,et al.  Phase-locking in the cochlear nerve of the guinea-pig and its relation to the receptor potential of inner hair-cells , 1986, Hearing Research.

[14]  The structure and probable function of the internal cuticular parts of Johnston's organ in mosquitoes (Aedes aegypti) , 1989 .

[15]  AC Tose.  Cell , 1993, Cell.

[16]  P. Belton.  Attraction of male mosquitoes to sound. , 1994, Journal of the American Mosquito Control Association.

[17]  M. Göpfert,et al.  Mosquito hearing: sound-induced antennal vibrations in male and female Aedes aegypti. , 1999, The Journal of experimental biology.

[18]  M. Göpfert,et al.  Nanometre–range acoustic sensitivity in male and female mosquitoes , 2000, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[19]  Daniel Robert,et al.  Active auditory mechanics in mosquitoes , 2001, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[20]  Mark Q Benedict,et al.  The first releases of transgenic mosquitoes: an argument for the sterile insect technique. , 2003, Trends in parasitology.

[21]  I. Russell,et al.  Flying in Tune: Sexual Recognition in Mosquitoes , 2006, Current Biology.

[22]  P. Belton,et al.  Attraction of female Uranotaenia lowii (Diptera: Culicidae) to frog calls in Costa Rica , 2006, The Canadian Entomologist.

[23]  Jorge Hendrichs,et al.  Area-wide control of insect pests: from research to field implementation. , 2007 .

[24]  R. Rosenfeld.  Nature , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.