Different activation of the endothelial L-arginine and cyclooxygenase pathway in the human internal mammary artery and saphenous vein.

The endothelium releases substances controlling vascular tone and platelet function. We investigated mediators of endothelium-dependent responses in human internal mammary arteries and saphenous veins. The inhibitor of nitric oxide formation, NG-monomethyl L-arginine, enhanced the sensitivity to norepinephrine (fivefold) and evoked more pronounced endothelium-dependent contractions in internal mammary arteries (19 +/- 6% of 100 mM KCl) than in saphenous veins (2 +/- 1%; p less than 0.005). In internal mammary arteries, NG-monomethyl L-arginine, but not indomethacin, markedly reduced endothelium-dependent relaxations to acetylcholine (from 95 +/- 2% to 39 +/- 7%; p less than 0.005) and prevented those to histamine (78 +/- 6% to 4 +/- 3%; p less than 0.005). In saphenous veins, endothelium-dependent relaxations to acetylcholine were weak (24 +/- 11%), while nitric oxide caused comparable relaxations (85 +/- 3%) as in internal mammary arteries (80 +/- 5%; NS). NG-Monomethyl L-arginine prevented the relaxations to acetylcholine and unmasked endothelium-dependent contractions (30 +/- 10%). Indomethacin and the thromboxane synthetase inhibitor CGS-13080 augmented relaxations of saphenous veins to acetylcholine from 24 +/- 11% to 46 +/- 9% (p less than 0.05). Histamine-evoked contractions were converted to endothelium-dependent relaxations by indomethacin and the thromboxane A2/endoperoxide receptor antagonist SQ-30741 (38 +/- 3% and 40 +/- 6%; p less than 0.05) but not CGS-13080. Thus, 1) nitric oxide mediates endothelium-dependent relaxations in human arteries and veins; 2) internal mammary arteries release more nitric oxide than do saphenous veins, and 3) in saphenous veins, the effects of nitric oxide are reduced by endothelium-derived contracting factors originating from the cyclooxygenase pathway.

[1]  P. Vanhoutte,et al.  Endothelium‐dependent hyperpolarization of canine coronary smooth muscle , 1988, British journal of pharmacology.

[2]  Z. Katušić,et al.  Superoxide anion is an endothelium-derived contracting factor. , 1989, The American journal of physiology.

[3]  S. Moncada,et al.  Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor , 1987, Nature.

[4]  Z. Katušić,et al.  Anoxic Contractions in Isolated Canine Cerebral Arteries: Contribution of Endothelium‐Derived Factors, Metabolites of Arachidonic Acid, and Calcium Entry , 1986, Journal of cardiovascular pharmacology.

[5]  J. Brunkwall,et al.  Prostacyclin and thromboxane release from the vessel wall--comparison between an incubation and a perfusion model. , 1987, Prostaglandins.

[6]  R. Furchgott,et al.  Role of endothelial cells in relaxation of isolated arteries by bradykinin. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[7]  H. Shimokawa,et al.  Impaired Endothelium‐Dependent Relaxation to Aggregating Platelets and Related Vasoactive: Substances in Porcine Coronary Arteries in Hypercholesterolemia and Atherosclerosis , 1989, Circulation research.

[8]  K. Okumura,et al.  Prostaglandin H2 may be the endothelium-derived contracting factor released by acetylcholine in the aorta of the rat. , 1990, Hypertension.

[9]  S. Moncada,et al.  ENDOGENOUS NITRIC OXIDE INHIBITS HUMAN PLATELET ADHESION TO VASCULAR ENDOTHELIUM , 1987, The Lancet.

[10]  J. Hibbs,et al.  Macrophage cytotoxicity: role for L-arginine deiminase and imino nitrogen oxidation to nitrite. , 1987, Science.

[11]  G. Rubanyi.  Endothelium‐derived relaxing and contracting factors , 1991, Journal of cellular biochemistry.

[12]  A. Weston,et al.  Endothelium-derived hyperpolarizing factor: a new endogenous inhibitor from the vascular endothelium. , 1988, Trends in pharmacological sciences.

[13]  L. V. von Segesser,et al.  Endothelium-derived relaxing factor and protection against contractions induced by histamine and serotonin in the human internal mammary artery and in the saphenous vein. , 1989, Circulation.

[14]  P. Vanhoutte,et al.  Heterogeneous Behavior of the Canine Arterial and Venous Wall: Importance of the Endothelium , 1982, Circulation research.

[15]  T. Lüscher,et al.  Endothelium-dependent contractions to acetylcholine in the aorta of the spontaneously hypertensive rat. , 1986, Hypertension.

[16]  S. Moncada,et al.  A specific inhibitor of nitric oxide formation from l‐arginine attenuates endothelium‐dependent relaxation , 1989, British journal of pharmacology.

[17]  T. Lüscher,et al.  Interaction Between Endothelium‐Derived Nitric Oxide and SIN‐1 in Human and Porcine Blood Vessels , 1989, Journal of cardiovascular pharmacology.

[18]  加藤 敏夫.  Prostaglandin H[2] may be the endothelium-derived contracting factor released by acetylcholine in the aorta of the rat , 1990 .

[19]  P. Néglen,et al.  The role of the endothelium in the control of venous tone: studies on isolated human veins. , 1988, Clinical physiology.

[20]  P. J. Simpson,et al.  Thromboxane synthetase inhibition with CGS 13080 improves coronary blood flow after streptokinase-induced thrombolysis. , 1987, American heart journal.

[21]  M. Marletta.  Nitric oxide: biosynthesis and biological significance. , 1989, Trends in biochemical sciences.

[22]  C. Nathan,et al.  Identification of arginine as a precursor of endothelium-derived relaxing factor. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[23]  V. Wiegand,et al.  Effect of acetylcholine on arterial and venous grafts and coronary arteries in patients with coronary artery disease. , 1990, European heart journal.

[24]  J. Vane,et al.  Differential formation of prostacyclin (PGX or PGI2) by layers of the arterial wall. An explanation for the anti-thrombotic properties of vascular endothelium. , 1977, Thrombosis research.

[25]  V. Fuster,et al.  Effect of dipyridamole and aspirin on late vein-graft patency after coronary bypass operations. , 1984, The New England journal of medicine.

[26]  Julie H. Campbell,et al.  Release and properties of endothelium‐derived relaxing factor (EDRF) from endothelial cells in culture , 1985, Journal of cellular physiology.

[27]  J. Bény,et al.  Neither nitric oxide nor nitroglycerin accounts for all the characteristics of endothelially mediated vasodilatation of pig coronary arteries. , 1988, Blood vessels.

[28]  W. C. Sheldon,et al.  Influence of the internal-mammary-artery graft on 10-year survival and other cardiac events. , 1986, The New England journal of medicine.

[29]  R. Furchgott,et al.  The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine , 1980, Nature.

[30]  V. Miller,et al.  Is nitric oxide the only endothelium-derived relaxing factor in canine femoral veins? , 1989, The American journal of physiology.

[31]  P. Vanhoutte.  The end of the quest? , 1987, Nature.

[32]  S. Moncada,et al.  A novel citrulline-forming enzyme implicated in the formation of nitric oxide by vascular endothelial cells. , 1989, Biochemical and biophysical research communications.

[33]  M. Bourassa,et al.  Comparison of late changes in internal mammary artery and saphenous vein grafts in two consecutive series of patients 10 years after operation. , 1984, Circulation.

[34]  A. Hassid,et al.  Nitric oxide-generating vasodilators and 8-bromo-cyclic guanosine monophosphate inhibit mitogenesis and proliferation of cultured rat vascular smooth muscle cells. , 1989, The Journal of clinical investigation.

[35]  J. T. Shepherd,et al.  Endothelium-dependent contractions to calcium ionophore A23187, arachidonic acid, and acetylcholine in canine basilar arteries. , 1988, Stroke.

[36]  J. Vane,et al.  Pharmacology and endogenous roles of prostaglandin endoperoxides, thromboxane A2, and prostacyclin. , 1978, Pharmacological reviews.

[37]  T. Lüscher,et al.  Endothelium-dependent regulatory mechanisms in human coronary bypass grafts: possible clinical implications. , 1989, Zeitschrift fur Kardiologie.

[38]  F. J. Fogerty,et al.  Regulation of histamine-mediated prostacyclin synthesis in cultured human vascular endothelial cells , 1981, Cell.

[39]  U. Förstermann,et al.  Selective Attenuation of Endothelium‐Mediated Vasodilation in Atherosclerotic Human Coronary Arteries , 1988, Circulation research.

[40]  E. Grabowski,et al.  Prostacyclin production by internal mammary artery as a factor in coronary artery bypass grafts. , 1986, Surgery.

[41]  T. Lüscher.  Endothelial Vasoactive Substances and Cardiovascular Disease , 1988 .

[42]  G E Reed,et al.  Early postoperative spasm in left internal mammary artery bypass grafts. , 1987, The Annals of thoracic surgery.

[43]  M. Ogletree,et al.  Leukotrienes cause mesenteric vasoconstriction and hemoconcentration in rats without activating thromboxane receptors. , 1989, Prostaglandins.

[44]  V. Miller,et al.  Endothelium-dependent contractions to arachidonic acid are mediated by products of cyclooxygenase. , 1985, The American journal of physiology.

[45]  S. Moncada,et al.  Actions of nitric oxide on the release of prostacyclin from bovine endothelial cells in culture. , 1988, European journal of pharmacology.

[46]  P. Vanhoutte,et al.  Hypoxia releases a vasoconstrictor substance from the canine vascular endothelium. , 1985, The Journal of physiology.

[47]  I. Leusen,et al.  Role of the endothelium in the vasodilator response of rat thoracic aorta to histamine. , 1983, European journal of pharmacology.

[48]  S. Moncada,et al.  Vascular endothelial cells synthesize nitric oxide from L-arginine , 1988, Nature.

[49]  V. Fuster,et al.  Noninvasive Radioisotopic Technique for Detection of Platelet Deposition in Coronary Artery Bypass Grafts in Dogs and Its Reduction with Platelet Inhibitors , 1979, Circulation.

[50]  L. V. von Segesser,et al.  Difference between endothelium-dependent relaxation in arterial and in venous coronary bypass grafts. , 1988, The New England journal of medicine.

[51]  N. Toda.  Mechanism of histamine actions in human coronary arteries. , 1987, Circulation research.

[52]  S. Moncada,et al.  The anti‐aggregating properties of vascular endothelium: interactions between prostacyclin and nitric oxide , 1987, British journal of pharmacology.