The Alteration Tissue Structure of Digestive diverticulum of Anodonta woodiana Induced by Lead

Accumulated the toxic compound such as lead in  A. woodiana  caused an alteration in tissue. The aim of the present study is observation through histology to determine the alteration of the structure of digestive diverticulum tissue.  A. woodiana  were maintained in water tub that treated lead II nitrate exposure (Pb(NO 3 ) 2 ) 0, 15, 25, 35 mgL -1  for 7 days to determine the alteration of the structure of damaged the digestive diverticulum and determining water quality such temperature, DO and pH. The highest damage occurs in digestive diverticulum treated 35 mgL -1 due to the many alteration tissue structure such as  hyperplasia, edema, atrophy, necrosis  with the percentage of total damage of about 35%. the lowest alteration tissue structure occurs in 15 mgL -1  treatment group of the digestive diverticulum. the temperature was observed at about 25.3– 26.4°C, pH about 6-7, and DO about 6.1-6.7 mgL -1 . Thus, the increment of lead dose treatment induces increment of tissue structure alteration in digestive diverticula tissue, therefore, might promote the death of  Anodonta woodiana. Keywords: Anodonta woodiana , digestive diverticulum, histology

[1]  Tslalom Haileslassie,et al.  Hazards Of Heavy Metal Contamination In Ground Water , 2015 .

[2]  S. Bullard,et al.  Histological Atlas of Freshwater Mussels (Bivalvia, Unionidae): Villosa nebulosa (Ambleminae: Lampsilini), Fusconaia cerina (Ambleminae: Pleurobemini) and Strophitus connasaugaensis (Unioninae: Anodontini) , 2014 .

[3]  J. Jebali,et al.  Comparative study of the bioaccumulation and elimination of trace metals (Cd, Pb, Zn, Mn and Fe) in the digestive gland, gills and muscle of bivalve Pinna nobilis during a field transplant experiment. , 2014, Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements.

[4]  R. Mcinnis,et al.  The impact of municipal wastewater effluent on field‐deployed freshwater mussels in the Grand River (Ontario, Canada) , 2014, Environmental toxicology and chemistry.

[5]  P. Vikesland,et al.  Uptake and retention of metallic nanoparticles in the Mediterranean mussel (Mytilus galloprovincialis). , 2013, Aquatic toxicology.

[6]  D. Arfiati,et al.  Pengamatan Jaringan Lambung Kijing Taiwan (Anodonta woodiana Lea) Yang Terdedah Pestisida Diazinon 60 EC Pada Beberapa Konsentrasi , 2013 .

[7]  Matthew E. Downs,et al.  The mechanics of the primary cilium: an intricate structure with complex function. , 2012, Journal of biomechanics.

[8]  J. Teyssie,et al.  Characterization of ²⁴¹ Am and ¹³⁴Cs bioaccumulation in the king scallop Pecten maximus: investigation via three exposure pathways. , 2011, Journal of environmental radioactivity.

[9]  D. Fattorini,et al.  Antioxidant, genotoxic and lysosomal biomarkers in the freshwater bivalve (Unio pictorum) transplanted in a metal polluted river basin. , 2010, Aquatic toxicology.

[10]  M. Delgado,et al.  First evidence of fiberglass ingestion by a marine invertebrate (Mytilus galloprovincialis L.) in a N.W. Mediterranean estuary. , 2009, Marine pollution bulletin.

[11]  Wen-Xiong Wang,et al.  Validation of biokinetic model of metals in the scallop Chlamys nobilis in complex field environments. , 2008, Environmental science & technology.

[12]  F. Regoli,et al.  Seasonal variations of exposure biomarkers, oxidative stress responses and cell damage in the clams, Tapes philippinarum, and mussels, Mytilus galloprovincialis, from Adriatic sea. , 2008, Marine environmental research.

[13]  J. Yeager,et al.  In Situ Toxicity Testing of Unionids , 2006 .

[14]  C. Gagnon,et al.  Exposure of caged mussels to metals in a primary-treated municipal wastewater plume. , 2006, Chemosphere.

[15]  M. Mahmoud,et al.  Ichthyophthiriasis: Various Fish Susceptibility or Presence of More than one Strain of the Parasite? , 2006 .

[16]  Agoes Soegianto,et al.  Pengaruh pemberian kadmium terhadap tingkat kelangsungan hidup dan kerusakan struktur insang dan hepatopankreas pada udang regang [Macrobrachium sintangense (de Man)] , 2004 .

[17]  E. Gurzău,et al.  Lessons from case studies of metals: investigating exposure, bioavailability, and risk. , 2003, Ecotoxicology and environmental safety.

[18]  D. Vélez,et al.  ARSENIC SPECIATION IN MANUFACTURED SEAFOOD PRODUCTS: A REVIEW , 1998 .

[19]  P. S. Pillai,et al.  HEAVY METAL TOXICITY IN BIVALVE- HISTOLOGICAL AND HISTOCHEMICAL ENQUIRY , 1993 .

[20]  F. Vernberg Marine Pollution and Physiology, Recent Advances , 1985 .

[21]  R. Hillman The American oyster,Crassostrea virginica gmelin , 1965 .

[22]  R. H.,et al.  A Medical Dictionary , 1918, Nature.